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Method for electrically and biologically rehabilitating soil adopting electrode matrix

An electrode matrix and electrokinetic biotechnology, applied in the restoration of polluted soil, etc., can solve the problems of low mass transfer rate, not yet involved in the field of in-situ bioremediation, inhibition, etc., and achieve the goal of strengthening the mass transfer process and improving the efficiency of bioremediation Effect

Inactive Publication Date: 2009-09-09
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

(2) In the soil with low pollutant concentration, the ability of microorganisms to utilize pollutants is inhibited
(3) Low mass transfer rate in soil medium due to physical and chemical effects such as adsorption and barrier
At present, the columnar electrode matrix is ​​only used to extract pollutants from soil, and has not yet been involved in the field of in situ bioremediation

Method used

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  • Method for electrically and biologically rehabilitating soil adopting electrode matrix
  • Method for electrically and biologically rehabilitating soil adopting electrode matrix
  • Method for electrically and biologically rehabilitating soil adopting electrode matrix

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Implementation example 1: enrichment of chlorophenols in soil

[0032] In the sandy loam layer, the moisture content is 17-19%. The initial concentration of 2,4-dichlorophenol was about 83mg / Kg dry soil, and a one-dimensional electrode configuration was used to apply a non-uniform electric field unidirectionally, with a voltage gradient of 1.0V / cm and an electrode spacing of 20cm. After the experiment, the concentration of 2,4-dichlorophenol was measured by sampling at different positions of the electrode pair connection. The chlorophenol in the soil sample was sent to high performance liquid chromatography for detection after three extractions with acetocyanide (the extraction rate reached 94.8%). The mobile phase consisted of methanol:5% glacial acetic acid aqueous solution=67:33, and the detection wavelength was 284nm.

[0033] image 3 It shows that when the test runs for 1 day, the content of 2,4-dichlorophenol at 1 cm away from the cathode has dropped to zero, w...

Embodiment 2

[0034] Implementation Example 2: Injection of Inorganic Nutrient Ion Nitrate

[0035]In the sandy loam layer, the water content is 17-19%, and NO is added in the fixed quartz sand area (C-r area) 3 - For ions, in a one-dimensional electrode configuration, a non-uniform electric field of 1.0V / cm is applied in one direction, and the electrode spacing is 100cm, and the experiment runs for 100 hours. A reaction system without voltage was used as a control. During the experiment, samples were taken every 10 hours, and the NO in different positions was measured by ion chromatography 3 - ion concentration.

[0036] Figure 4 Indicates that during 100 hours of one-way operation, NO 3 - The ions are constantly migrating towards the anode and diffuse throughout the soil area. After 80 hours, NO 3 - The ion concentration reached or was close to 1.4mg / Kg soil, while no NO was detected at the sampling point in the control experiment 3 - ions, which confirms the adoption of this...

Embodiment 3

[0037] Implementation Example 3: Injection of Phenol Efficient Degradation Bacteria

[0038] In the sterilized sandy loam layer, the moisture content is 17-19%. Add a high concentration of bacterial suspension in the quartz sand area (C-m area). In the one-dimensional electrode configuration, a non-uniform electric field of 1.0 V / cm was applied unidirectionally, the electrode spacing was 20 cm, and the experiment was run for 32 hours. A reaction system without voltage was used as a control. During the experiment, samples were taken to determine the number of live and dead bacteria in the soil samples. The soil samples were extracted by shaking with sodium pyrophosphate and counted. The number of live bacteria was counted by the plate technique (CFU), and the number of dead bacteria was stained with the Baclight LIVE / DEAD probe of Molecular Probes.

[0039] After 20 and 28 hours of operation, the bacterial concentration at a0 and c0 respectively reached the maximum value, bu...

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Abstract

The method for electrodynamically bioremediating soil by using an electrode matrix belongs to the technical field of environmental protection, and in particular relates to the technical field of repairing soil by using an electrode matrix. It is characterized in that it uses a one-dimensional or two-dimensional electrode matrix, and adopts a one-way operation mode to disperse foreign substances into the soil; a two-way operation mode is used to enhance the mass transfer between pollutants and degrading bacteria, and perform direct in-situ strengthening . The invention can effectively enrich the pollutants in the soil to a specific treatment area; can quickly disperse the fixed-point injected nutrients and microorganisms into the soil; can strengthen the mass transfer process of pollutants and degrading bacteria in the soil, Improve bioremediation efficiency.

Description

technical field [0001] The invention belongs to the technical field of environmental protection, in particular to the technical field of repairing soil by using an electrode matrix. Background technique [0002] Compound organic pollution is one of the most serious problems in soil pollution. There are 13-16 million hm across the country 2 Cultivated land is polluted with pesticides. Due to the pollution of aromatics, alkanes, olefins, phenols, acids, aldehydes, ketones, esters and other organic substances in the oilfield chemical industry area, the concentration of pollutants in the soil is often tens to thousands of times the background value. my country's population density is high, and its dependence on land far exceeds that of other countries, so the task of remediation of contaminated soil is arduous. [0003] Organic Contaminated Soil Bioremediation Technology Including Ex-Situ Biological Treatment [0004] (such as bio-slurry reactors, etc.) and in-situ bioremedi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B09C1/00B09C1/10
Inventor 王慧范向宇罗启仕张锡辉
Owner TSINGHUA UNIV
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