Digital-micro-mirror-device-based fast precise optical focusing enhancement method and system

Abstract

The invention discloses a digital-micro-mirror-device-based fast precise optical focusing enhancement method and system. Region division is carried out on a digital micro mirror device and the digital micro mirror device is divided into two parts evenly; divided areas in the second part are kept to be unchanged and intensity modulation is carried out simultaneously on light beams at all divided areas in the first part at different frequencies, fluorescent light with changed intensities is excited after light beam focusing, and the excited fluorescent light is received and recorded by a photomultiplier; after Fourier transform on a fluorescent light signal, compensation phases corresponding to all divided areas are obtained; the divided areas in the first part are kept to be unchanged d intensity modulation is carried out simultaneously on light beams at all divided areas in the second part to obtain another halves of compensation phases; the divided areas with the phase values smaller than pai are kept and the divided areas with the phase values larger than pai are removed to obtain screened divided areas; and after the digital micro mirror device loads the screened divided areas, the light beams are focused in a sample to form a light spot with the higher central light intensity. On the basis of the optical diffraction principle, the beam focusing speed is increased and the light beam focusing capability is enhanced. A feasible focused light spot generation method can be provided for the non-fiber interpolation type optogenetics and the deep penetration microimaging.

Description

technical field [0001] The invention belongs to the field of optogenetics and optical microscopic imaging, and in particular relates to a fast and precise optical focusing enhancement method and system based on a digital micromirror device, which is applied to optogenetic light stimulation and optical microscopic imaging. Background technique [0002] In the field of biomedical optics, optical scattering is the main factor restricting the quality of optical imaging. Most optical techniques for deep tissue imaging (eg, confocal laser imaging, two-photon microscopy, and optical coherence tomography) primarily utilize non-scattered photons (ie, ballistic photons) for imaging. The number of ballistic photons decays exponentially with depth, thus limiting the beam focus to a depth of 1 mm. [0003] Optogenetic technology requires photostimulation of specific neurons to study its neural circuit mechanism. However, the traditional optical fiber implantable optogenetics has seriou...

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Problems solved by technology

[0008] In order to solve the problems existing in the background technology, the purpose of the present invention is to use a digital micromirror device with a higher image refresh rate to solve the problem of spatial light modulation in traditional adaptive optogenetics The problem of taking a long time

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