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Preparation method of anti-wrinkle anti-oxidation biological mask and biological mask product

A biological facial mask and anti-oxidation technology, applied in biochemical equipment and methods, cosmetic preparations, cosmetics, etc., can solve problems such as limited effect, partial or complete failure of nutrients, and impact on efficacy

Inactive Publication Date: 2017-10-10
北京众兴华纳生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] To sum up, the effect of bio-masks on the market on skin beauty is very limited. Although some nutrients are added to them, these nutrients have been partially or completely lost due to storage conditions.
In addition, the method of using the biological mask also greatly affects its efficacy.

Method used

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  • Preparation method of anti-wrinkle anti-oxidation biological mask and biological mask product
  • Preparation method of anti-wrinkle anti-oxidation biological mask and biological mask product
  • Preparation method of anti-wrinkle anti-oxidation biological mask and biological mask product

Examples

Experimental program
Comparison scheme
Effect test

preparation example 1

[0048] Preparation Example 1 Preparation of human autologous platelet-rich plasma

[0049] Use 10ml EDTA anticoagulant blood collection tubes (BD Company, USA) to extract 100ml anticoagulant meridian blood, centrifuge 10-80ml plasma at 1000 rpm for 10 minutes from 10 10ml anticoagulant tubes, and centrifuge again at 3000 rpm for 10 minutes Obtain 40ml of platelet-rich plasma and store it in a 4°C refrigerator for later use.

preparation example 2

[0050] Preparation Example 2 Acquisition of Human Autologous Stromal Stem Cells

[0051] Take 60ml of blood cells obtained from autologous platelet-rich plasma by centrifugation in Preparation Example 1, dilute them with 60ml of 1×PBS (pH 7.4), add them to the lymphatic separation solution, and undergo density gradient centrifugation at 1500rpm for 25min to obtain mononuclear cells. MEM medium (containing 5% fetal bovine serum, 50ng / ml recombinant human insulin-like growth factor-1, 10ng / ml recombinant human stem cell growth factor, 20ng / ml epidermal growth factor and 20ng / ml recombinant human basic fibroblast growth factor, American Paptek Corporation) resuspended, and the staining count of Moss Panlan was 2.4×10 8 cells, by 5×10 6 Cells / ml, add to 75cm 2 12ml in culture flasks, 4 flasks in total, at 37°C, 5% CO 2 Grow in the incubator for 48 hours.

[0052] After 48 hours, replace the medium for the cultured stromal stem cells, discard the culture medium with a 10ml pipe...

Embodiment 3

[0055] The preparation of embodiment 3 autologous gel-like bio-masks

[0056] Take 2 grams of collagen powder into a 250ml sterile beaker, add 40ml of 70°C normal saline, stir to dissolve fully, add 20ml of prepared autologous platelet-rich plasma when the temperature drops to about 40°C, mix well and add the prepared 1ml The stromal stem cell liquid, after continuing to mix, is added to the mask mold, placed in the refrigerator at 4°C to form a gel-like bio-mask and stored. figure 2 Picture of the prepared autologous gel-like bio-mask.

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Abstract

The invention provides a preparation method of an anti-wrinkle anti-oxidation biological mask. The method includes the steps: acquiring platelet-rich plasma and mesenchymal stem cells from autologous peripheral blood; adding the platelet-rich plasma and the mesenchymal stem cells into collagen liquid to form a gel-like or semi-gel-like mask; adding growth factors and anti-oxidation molecules to prepare the autologous biological mask product. The biological mask can be used for reducing wrinkles of skins, resisting oxidation, removing couperose skins, removing speckles, whitening skins and the like.

Description

technical field [0001] The invention relates to a method for preparing a wrinkle-removing and anti-oxidation biological mask and a biological mask product obtained by the method. In particular, the present invention relates to the use of autologous peripheral blood-derived platelet-rich plasma and stromal stem cells, added to collagen to form a gel-like or semi-gel-like mask, and growth factors and antioxidant molecules can be added at the same time, and through the The biological facial mask product that described method obtains. The biological facial mask of the invention can be used for medical cosmetology, and has the effects of wrinkle removal, anti-oxidation, redness removal, spot removal, whitening and the like on the skin. Background technique [0002] At present, there are various biological facial mask products on the market, which have formed a popular product generally accepted by people. The vast majority of biological masks use mainly hydrogels prepared from ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/98A61K8/99A61K8/65A61K8/64A61K8/66A61K8/9771A61K8/02A61K8/04A61Q19/08A61Q19/00A61Q19/02C12N5/0789
CPCA61K8/0212A61K8/042A61K8/64A61K8/65A61K8/66A61K8/97A61K8/982A61K8/983A61K8/99A61Q19/00A61Q19/02A61Q19/08C12N5/0634C12N2501/105C12N2501/11C12N2501/115C12N2501/12
Inventor 宋静
Owner 北京众兴华纳生物科技有限公司
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