Culture medium for cultivating Antrodia sporocarp and cultivating method thereof
A technology of Antrodia cintrodia and culture medium, which is applied in the field of edible and medicinal fungi, can solve the problems of high price, Antrodia cinnamomea is not the future development direction, and scarce wild resources, and achieve the effects of shortening the growth time, not being easily infected by miscellaneous bacteria, and reducing the pollution rate
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Embodiment 1
[0024] The present embodiment provides a kind of method of cultivating Antrodia camphorata fruiting bodies, and described scheme is carried out according to the following steps:
[0025] S01: Obtain strains: purchase Antrodia camphorata strains and store them on PDA slant medium;
[0026] S02: Preparation of liquid strains: 150ml of liquid seed culture medium was added to a 500ml Erlenmeyer flask, and then activated Antrodia camphorata strains were inserted, and the rotary shaker was cultivated at a temperature of 23°C under dark conditions for 10 days to obtain liquid Antrodia camphorata strains;
[0027] S03: Preparation of cultivation medium: make oak wood stakes with a diameter of 20 cm and a length of 20 cm, put them in water and soak them for 30 days, add lignin, glucose, corn flour and potassium dihydrogen phosphate during the soaking process, and the soaking is completed Finally, clean the bark and put it into a polypropylene 500*500 bacteria bag, cover the mouth of t...
Embodiment 2
[0032] The present embodiment provides a kind of method of cultivating Antrodia camphorata fruiting bodies, and described scheme is carried out according to the following steps:
[0033] S01: Obtain strains: purchase Antrodia camphorata strains and store them on PDA slant medium;
[0034] S02: Preparation of liquid strains: Add 150ml of liquid seed culture medium into a 500ml Erlenmeyer flask, then insert activated Antrodia camphorata strains, and cultivate in a rotating shaker at a speed of 160r / min for 15 days to obtain liquid Antrodia camphorata strains;
[0035] S03: Preparation of cultivation medium: make oak wood stakes with a diameter of 20 cm and a length of 20 cm, put them in water and soak them for 30 days, add lignin, glucose, corn flour and potassium dihydrogen phosphate during the soaking process, and the soaking is completed Finally, clean the bark and put it into a polypropylene 500*500 bacteria bag, cover the mouth of the bacteria bag with a plastic tube with ...
Embodiment 3
[0040] The present embodiment provides a kind of method of cultivating Antrodia camphorata fruiting bodies, and described scheme is carried out according to the following steps:
[0041] S01: Obtain strains: purchase Antrodia camphorata strains and store them on PDA slant medium;
[0042] S02: Preparation of liquid strains: Add 150ml of liquid seed culture medium into a 500ml Erlenmeyer flask, then insert activated Antrodia camphorata strains, rotate the shaker, rotate at 160r / min, and cultivate in the dark at a temperature of 26°C for 12 days to obtain liquid Antrodia camphorata strains;
[0043] S03: Preparation of cultivation medium: make oak wood stakes with a diameter of 20 cm and a length of 20 cm, put them in water and soak them for 30 days, add lignin, glucose, corn flour and potassium dihydrogen phosphate during the soaking process, and the soaking is completed Finally, clean the bark and put it into a polypropylene 500*500 bacteria bag, cover the mouth of the bacter...
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