Unlock instant, AI-driven research and patent intelligence for your innovation.

A kind of method for measuring the efficacy of mycoplasma hyopneumoniae vaccine

A technology for the determination of Mycoplasma hyopneumoniae and its detection method, which is applied in the field of determination of Mycoplasma hyopneumoniae vaccine efficacy and vaccine efficacy. The results are intuitive and reliable, the detection cycle is shortened, and the effect is conducive to general promotion

Active Publication Date: 2020-04-03
RINGPU (BAODING) BIOLOGICAL PHARMACEUTICAL CO LTD
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage of piglet immune challenge method is that the virus attack requires a negative pressure environment, the attack technology is difficult to master, and each time the test pig needs to be dissected to calculate the reduction rate of lung lesions, which is subject to subjective influence, long detection cycle, and high cost. The repeatability is poor; the rabbit IHA assay uses rabbits as experimental animals, which are non-target animals, and cannot accurately reflect the true efficacy of the vaccine; the ELISA antibody detection method is used to determine the relative efficacy of the vaccine, and the ELISA detection method is affected by the differences between batches of kits , poor reproducibility, and many literatures have reported that ELISA antibody levels are not correlated with vaccine protection levels

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of method for measuring the efficacy of mycoplasma hyopneumoniae vaccine
  • A kind of method for measuring the efficacy of mycoplasma hyopneumoniae vaccine
  • A kind of method for measuring the efficacy of mycoplasma hyopneumoniae vaccine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 Comparison test of efficacy of Mycoplasma hyopneumoniae vaccine

[0038] The same batch of Mycoplasma hyopneumoniae inactivated vaccine (J strain) was detected simultaneously using the method of the present invention, the ELISA antibody detection method and the piglet immune challenge method respectively, and the detection results of the three methods were compared.

[0039] Inactivated Mycoplasma hyopneumoniae vaccine (J strain): produced by Ruipu (Baoding) Biopharmaceutical Co., Ltd., 10 doses / bottle, available on the market.

[0040] 1. Immunize animals

[0041] Select 6 groups of healthy piglets with negative Mycoplasma hyopneumoniae antigen and antibody from 7 to 14 days old, 5 pigs / group, each neck muscle injects 2ml of inactivated Mycoplasma hyopneumoniae vaccine (J strain), 2 weeks after the first vaccination with the same dosage and method Perform 2 exemptions. Set the non-immune group to control 6 groups, 5 heads / group.

[0042] 2. Efficacy determination

[0...

Embodiment 2

[0078] Example 2 The method of the present invention measures the efficacy of various Mycoplasma hyopneumoniae vaccines

[0079] Vaccine 1: Inactivated Mycoplasma hyopneumoniae vaccine (J strain); Vaccine 2: Live Mycoplasma hyopneumoniae vaccine (168 strains); Vaccine 3: Inactivated Mycoplasma hyopneumoniae vaccine (P-5722-3 strain); Vaccine 4: Swine pneumonia Live Mycoplasma Vaccine (RM48 strain). Four vaccines are available in the market.

[0080] (1) Immunize animals and collect serum

[0081] Vaccinate healthy piglets 7 to 14 days old with negative Mycoplasma hyopneumoniae antigen antibody according to the instruction manual of each commercial Mycoplasma hyopneumoniae vaccine. 28 days after the last immunization, the jugular vein blood was collected aseptically to separate the serum. Set up a blank control group.

[0082] (2) Effectiveness test

[0083] With the method of the present invention in Example 1, the four Mycoplasma hyopneumoniae vaccines of different strains were dete...

Embodiment 3

[0090] Example 3 The influence of different judgment time on the result of the method of the present invention

[0091] The 96-well cell plate with the determined result in Example 2 was placed in a 37°C constant temperature incubator and continued to be cultured until 21 days, and the results were recorded every 24 hours. The results are shown in Table 6

[0092] Table 6 The influence of different judgment times on the results

[0093]

[0094] Note: Each serum titer data in the above table represents the average titer of a group of serum samples.

[0095] The results showed that all wells did not change color for 1 to 5 days after culture, and the serum titer of vaccine 1 to 4 was 2 5 , The result cannot be judged; cultured for 6-7 days, the positive wells are all discolored, the serum titer of vaccine 1 to 4 is obviously different, and the result can be determined; cultured for 8 to 14 days, the serum titer of vaccine 1 to 4 is 2 0 After culturing for 15-21 days, the serum titers o...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for measuring an effect of a mycoplasma hyopneumoniae vaccine, and belongs to the field of biotechnology. The method comprises the following steps: (1) immunizing an animal; (2) collecting and diluting blood serum; (3) combining and culturing mycoplasma and the blood serum; (4) judging a result. The method disclosed by the invention uses a serological method to replace an animal experiment, and is easy to operate, low in technical requirement, strong in repeatability and good in specificity, and the establishment of the method avoids subjectivity of determination of a result of an immunogenicity and protective efficiency method, avoids toxin diffusion, and provides a basis for making and optimizing piglet immune procedure, so that the method is suitable for universal promotion.

Description

Technical field [0001] The invention relates to a method for determining the efficacy of a vaccine, in particular to a method for determining the efficacy of a Mycoplasma hyopneumoniae vaccine, belonging to the field of biotechnology. Background technique [0002] Swine enzootic hyopneumoniae (Swine enzootic hyopneumoniae), commonly known as swine asthma in my country, is a chronic, contact, and respiratory infectious disease caused by Mycoplasma hyopneumoniae (Mh). The main clinical manifestations are cough, asthma, and pig infections. Later, growth and development are often delayed, and the feed conversion rate is severely reduced, resulting in significant economic losses. Moreover, once a pig is infected with Mycoplasma, it is very easy to be infected by other viruses and bacteria, which will lead to an increase in mortality. Domestic and foreign data and a large amount of clinical data show that vaccine immunization is the most important means to prevent this disease. [0003]...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/569
CPCG01N33/56933
Inventor 郑朝朝张德宝高晓磊刘涛郁宏伟朱秀同柳珊杨保收
Owner RINGPU (BAODING) BIOLOGICAL PHARMACEUTICAL CO LTD
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com