Double-drive coupled operation chip device for joint detection of six typical tumor markers

Abstract

The invention relates to a double-drive coupled operation chip device for joint detection of six typical tumor markers, belonging to the field of analytical tests. The application of polydimethylsiloxane (PDMS) in preparation of a substrate of a micro-fluid chip for multichannel joint detection of the six typical tumor markers has obvious advantages, but still has a series of problems; the chip device aims at solving the series of problems. The chip device is characterized in that the PDMS having an originally ecological surface is selected as the substrate, and a minitype ultrasonic transducer is fixedly arranged at the position close to a sample liquid stream terminal of the micro-fluid chip; meanwhile, an ultrasonic attenuator is arranged at a sample inlet end of the micro-fluid chip, so that the ultrasonic strength decreases rapidly and progressively in a short distance; therefore, an interfacial tension difference is formed at the two ends of the chip; the interfacial tension difference provides a force which drives a sample liquid stream to flow to the terminal along a hydrophobic capillary channel, and the force has coupled and cooperative operation with mechanical pumping force of a micropump in the structure at the same time.

Description

technical field [0001] The invention relates to a chip device for joint detection of six typical tumor markers with dual-drive coupling operation, belonging to the field of analysis and testing. Background technique [0002] Tumor markers (tumor markers, TM) refer to a class of substances produced by tumor cells themselves or produced by the body's response to tumor cells during the occurrence and proliferation of tumors, reflecting the existence and growth of tumors, including Proteins, hormones, enzymes (isoenzymes) and oncogene products, etc. Testing the tumor markers in the patient's blood or body fluids can detect tumors early in the tumor screening, observe the curative effect of tumor treatment and judge the prognosis of patients. Currently, the tumor markers commonly used clinically are: (1) alpha-fetoprotein (AFP) is a marker for primary liver cancer, testicular cancer, ovarian cancer and other tumors; (2) carcinoembryonic antigen (CEA) is a marker for digestive sy...

AI Technical Summary

Problems solved by technology

[0008] But it's not that simple

[0009] First, this polydimethylsiloxane material, the material referred to by the acronym PDMS, is itself a strongly hydrophobic material. Microchannels are built on this material. If the microchannels are not targeted The modification operation of the surface of the channel, then, after the overall assembly is completed, that is, after the cover is covered, because the inner surface of the micro channel in the structure occupies most of the inner surface of the liquid flow channel, then the PDMS micro channel The strong hydrophobic characteristic of the inner surface of the channel is the decisive factor, which will make it very difficult for the polar liquid flow similar to the aqueous solution to pass through, and its flow resistance is so large that even ordinary micropumps are difficult to push. Of course, If the cover sheet also chooses to use the PDMS material, then the problem is basically the same, with little difference; therefore, in the prior art, it is necessary to modify and modify the inner surface of the microchannel on the PDMS material; then , is this modification operation for the inner surface of the PDMS microchannel very troublesome? That's not the problem. What constitutes a serious technical problem is another problem: the PDMS polymer molecules in the bulk phase of the PDMS material substrate have the characteristics of automatic diffusion and migration to the surface. The characteristics of polymer molecules diffusing and migrating to the surface automatically will make the modified state of the inner surface of the microchannel modified by the surface modification unable to maintain for a long enough time, and the microgroove after surface modification The maintenance time of the inner surface state of the channel is roughly only enough to complete the time required for the internal test experiment in the laboratory; in other words, the inner surface of the PDMS microchannel after surface modification or surface modification is formed after modification The surface state of the surface does not last long, but quickly tends to or changes back to the surface state before the surface modification, and returns to the original strongly hydrophobic surface state in a relatively short period of time. Then, just imagine, Can such microfluidic chips be produced in large quantities, stored in large quantities, and widely promoted? The answer is obvious, that is, impossible

[0017] Third, as mentioned above, the inner surface of the PDMS microchannel is strongly hydrophobic, and targeted surface chemical modification or surface chemical modification is difficult to last. It is effective to use it within a short period of time; if the relatively short expiration date has passed and it is still used forcibly, since the surface state is already close to the hydrophobic state, there must be a comparison between using the conventional micropump to drive the sample liquid flow. Large flow resistance, in this way, the sample liquid can only be forced to flow in the target direction by increasing the pumping power and pumping pressure of the micropump. Pumping pressure to pump the sample liquid flow will cause bubbling, puffing, twisting, and deformation of the microchannels at the sampling end of the substrate including the area near the sampling end, and, under such high pressure conditions, The microgroove and its periphery at the sample inlet and its vicinity are also prone to peeling between the substrate and the cover slip. In this case, the sample solution will enter between the substrate and the cover slip formed after the peeling. This actually leads to the damage of the microfluidic chip; of course, if the surface modification or the surface modification is not in place, it will also lead to the above-mentioned situation within the short customary validity period; In the case of simply using an external micropump for liquid flow drive, the above-mentioned problem always exists

As mentioned above, if no pre-operations such as surface modification or surface modification have been done at all, then the above-mentioned problem will be more serious, even if the microchannel bubbling at the injection end and its vicinity does not occur , puffing, twisting, deformation, and peeling between the substrate and the cover sheet, etc., just because the flow resistance is too large, the use of a high-pressure micropump may not be able to drive the sample liquid flow toward the terminal.

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