Application of cyclic dipeptide cyclo-(S)-pro-(S)-phe to prevention and control of bacterial wilt and/or rice blast

A cyclic dipeptide and bacterial wilt technology, which is applied in the field of plant disease prevention and control, can solve the problems of destroying ecological balance, polluting the environment, and pathogenic bacteria producing drug resistance, and achieves the effect of simple steps, simple operation, and obvious inhibitory effect

Inactive Publication Date: 2017-12-15
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the prevention and control methods of bacterial wilt mainly include strengthening field management, cultivating disease-resistant varieties, using chemical agents, and applying biocontrol agents, etc., but practice has proved that field management i

Method used

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  • Application of cyclic dipeptide cyclo-(S)-pro-(S)-phe to prevention and control of bacterial wilt and/or rice blast
  • Application of cyclic dipeptide cyclo-(S)-pro-(S)-phe to prevention and control of bacterial wilt and/or rice blast
  • Application of cyclic dipeptide cyclo-(S)-pro-(S)-phe to prevention and control of bacterial wilt and/or rice blast

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] 1. Strain and culture medium

[0035] Escherichia coli (Escherichia coli) GZ-34, whose preservation number is CCTCC NO:M 2016353, was preserved in the Chinese Type Culture Collection Center located in Wuhan University, Wuhan, China on June 27, 2016, and has applied for a patent in China CN 105969707 A discloses.

[0036] Ralstonia solanacearum GMI1000, purchased from ATCC.

[0037] LB solid medium: tryptone 10g, yeast extract 5g, NaCl 10g, agar 15g, H 2 Dilute to 1000ml with O, pH 7.0-7.2. Sterilize at 121°C for 20 minutes.

[0038] LB liquid medium: The only difference from LB solid medium is that it does not contain agar.

[0039] Preparation of TTC solid medium: tryptone 10g, acid hydrolyzed casein 1g, glucose 5g, agar 15g, H 2 Dilute O to 1000ml, pH 6.8-7.2; sterilize at 121°C for 20 minutes to obtain basic medium. TTC (2,3,5-triphenyltetrazolium chloride) was made into a 0.1% (w / v) solution with distilled water and sterilized by filtration with a bacterial fil...

Embodiment 2

[0061] Embodiment 2: the antibacterial activity assay of cyclic dipeptide

[0062] 1. Medium

[0063] TTC culture medium: same as embodiment 1.

[0064] TTC liquid culture medium: with embodiment 1.

[0065] Rice bran medium: rice bran 20g, yeast extract 2g, agar 11g, H 2 Dilute O to 1000ml, pH 6.8-7.2. Sterilize at 121°C for 20 minutes.

[0066] 2. Experimental steps

[0067] 2.1 Culture medium preparation

[0068] Same as example 1.

[0069] 2.2 Activation of strains

[0070] Same as example 1.

[0071] 2.3 Activity detection

[0072] Pick a single colony of R. solanacearum and inoculate it in 10mL of TTC liquid medium, and culture it with shaking at 220r / min at 28°C for one day, then add 0.1mL of R. solanacearum liquid to 10mL of melted TTC solid medium (cool to about 45°C ) into a flat plate, and after solidification, use a sterile puncher (8 mm in diameter) to punch holes in the center of the plate containing bacteria, one hole per plate, take out the bacterial b...

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Abstract

The invention discloses application of cyclic dipeptide cyclo-(S)-pro-(S)-phe to prevention and control of bacterial wilt and/or rice blast. The inventor finds out that the cyclic dipeptide cyclo-(S)-pro-(S)-phe has a very strong inhibition effect on rastonia solanacearum and provides a new option for preparation of a novel bacterial wilt biocontrol preparation for the first time; furthermore, the cyclic dipeptide cyclo-(S)-pro-(S)-phe also has a remarkable inhibition effect on spores of pyricularia grisea. A preparation method of the cyclic dipeptide cyclo-(S)-pro-(S)-phe, provided by the invention, takes escherichia coli GZ-34 as a strain, and comprises the steps of culturing, separating and purifying; the method comprises the simple steps and is simple to operate, so that industrial production is easy to carry out.

Description

technical field [0001] The invention belongs to the field of plant disease prevention and control, and particularly relates to the application of cyclo-(S)-pro-(S)-phe in the prevention and treatment of bacterial wilt and / or rice blast. Background technique [0002] Plant bacterial wilt is a destructive soil-borne bacterial disease of vascular bundles caused by Ralstonia solanacearum. It is a devastating soil-borne disease with widespread systemic infection in subtropical, tropical and temperate regions, commonly known as "Plant Plague". R. solanacearum has a wide range of hosts and can infect more than 450 species of plants in 54 families. At present, the prevention and control of bacterial wilt is a major problem recognized in the world. It is widely distributed all over the world, and it occurs seriously in southern provinces and cities in my country, especially in Zhejiang. In recent years, not only Solanaceae and melons have been damaged, but also large-scale Mulberry ...

Claims

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Application Information

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IPC IPC(8): A01N37/46A01P3/00C12P21/04C12R1/19
CPCA01N37/46C07K5/12C12P21/02
Inventor 邓音乐宋施豪付书娜孙秀云邓海飞叶佩仪
Owner SOUTH CHINA AGRI UNIV
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