An intelligent system based on hrm technology

Abstract

The invention claims an HRM technology based intelligent system. The HRM technology based intelligent system comprises a sample preprocessing module, a temperature control module, a material adding module, a progress detection module and a control-analysis module, wherein the sample preprocessing module, the temperature control module, the material adding module and the progress detection module are connected with the control-analysis module respectively, the sample preprocessing module is used for collecting and preparing samples, and comprises a sample collection unit, a sample detection unit, a sample determination unit and a sample dilution unit, the temperature control module is used for controlling the temperature of a reaction system, and the temperature control module comprises a temperature detection module, a temperature determination module, a temperature presetting module and a temperature regulation module. The intelligent system provided by the invention has a reasonable design and high detection efficiency and accuracy for the samples, and the sample concentration and temperature and sample adding are all controlled intelligently to avoid personal errors.

Description

technical field [0001] The invention relates to the technical field of biological testing, in particular to an intelligent system based on HRM technology. Background technique [0002] High-resolution melting curve analysis technology, referred to as HRM, is a new tool for detecting gene mutations, genotyping and SNP detection that has emerged in recent years. It can quickly detect single-base mutations in nucleic acid fragments. HRM technology has been rapidly applied in research work in the fields of life science, medicine, agronomy, animal husbandry and other fields due to its advantages of simple operation, rapidity, high throughput, high sensitivity and specificity, no pollution to samples, and low cost of use. . In particular, it has broad application prospects in gene mutation scanning, genome matching, allele frequency analysis, species and variety identification, methylation research, forensic science and paternity testing. [0003] However, there are still some f...

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Problems solved by technology

[0003] However, there are still some factors that affect the detection results in the practical application of HRM technology, such as the control of sample concentration, the concentration of DNA fragments obtained from samples with different concentrations is also different, and DNA fragments with different concentrations will melt them The temperature value changes, so the concentration of the sample will also affect the accuracy. In practical applications, manual dilution is usually used, resulting in low sample preparation efficiency and large concentration preparation errors; Whether the amplification product reaches the plateau will also affect the test results of the sample. In practical applications, the number of amplification cycles is usually increased in order to reach the plateau of as many amplification products as possible, and too many cycles will cause amplification. The extension of time and the reduction of reaction efficiency

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