Composite microorganism composition and feed for piggies at lactation period
A compound microorganism and composition technology, applied in the field of compound microorganism composition and feed, can solve the problems of ineffective weight gain, reduction of diarrhea rate and mortality rate, poor effect, etc., and achieves strong synergistic effect, reasonable proportion, Effect of increasing average daily weight gain
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Embodiment 1
[0069] Prepare the microbial composition with reference to the following methods:
[0070](1) Preparation of Lactobacillus plantarum subsp.plantarum (Lactobacillus plantarum subsp.plantarum) Zhang-LL (CGMCC No.6936) freeze-dried bacterial powder: ① Strain activation: L.plantarum Zhang-LL strain in glycerol storage tube according to 2 % inoculum amount inoculated into 10mL sterilized improved MRS culture medium, and cultured at 37°C for 12 hours; ②Strain fermentation: Inoculate the bacterial liquid obtained from the above culture into 100mL sterilized improved MRS culture medium according to 2% inoculum amount, and other culture conditions were the same as above ; ③Strain fermentation: inoculate 1 000mL sterilized and improved MRS culture solution with the above-mentioned expanded culture solution according to 2% inoculation amount, and the fermentation time is 16h, and other culture conditions are the same as above; ④Thalline collection and freeze-drying: after the fermentation...
Embodiment 2
[0074] Prepare the microbial composition with reference to the method described in Implementation 1, the only difference is that the viable counts of L.plantarum Zhang-LL and B.coagulans Liu-g1 in the microbial composition are 1.0×10 9 CFU / mL.
Embodiment 3
[0076] Prepare the microbial composition with reference to the method described in Implementation 1, the only difference is that the viable counts of L.plantarum Zhang-LL and B.coagulans Liu-g1 in the microbial composition are 1.0×10 10 CFU / mL.
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