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Three-flavor lactic acid bacteria for ensiling of alpine postoral area

A lactic acid bacteria, pastoral technology, applied in bacteria, applications, animal husbandry and other directions, can solve the problems of lack of technology, limited production capacity, tight capital and other problems, and achieve the effect of improving digestibility, improving fermentation quality and fast growth rate

Inactive Publication Date: 2018-01-19
SICHUAN ACAD OF GRASSLAND SCI
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Problems solved by technology

At the same time, in the alpine pastoral areas of Northwest Sichuan, especially in remote, economically underdeveloped, and technologically backward areas, due to the constraints of objective conditions such as lagging equipment, tight funds, lack of technology, and limited production capacity, the use of mechanical equipment in the silage preparation process is promoted. It is not practical to increase the storage temperature to improve the quality of silage

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  • Three-flavor lactic acid bacteria for ensiling of alpine postoral area
  • Three-flavor lactic acid bacteria for ensiling of alpine postoral area
  • Three-flavor lactic acid bacteria for ensiling of alpine postoral area

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Embodiment Construction

[0023] 1Materials and methods

[0024] 1.1 Separation of lactic acid bacteria

[0025] A total of 86 samples of natural silage (old awn wheat, oats) were collected from the alpine pasturing area of ​​northwest Sichuan, and they were placed in a sterile ziplock bag with sterile tweezers, sealed, and transported to the laboratory in a car refrigerator, and stored at -80°C for later use.

[0026] Place the frozen sample on an ultra-clean workbench to thaw at room temperature, take 5g of the sample in a 250mL sterile Erlenmeyer flask, add 45mL 0.9% sterile saline, seal with plastic wrap, shake for 2 to 3 hours, filter with sterile gauze, and take the filtrate 1m for gradient dilution, respectively take 10 -3 , 10 -4 , 10 -5 1 mL of the diluted sample solution was spread in a sterile medium. Coat 3 to 5 plates (Φ10cm) for each dilution. The medium is MRS medium and CaCO 3 +MRS complex medium, M17 medium and GYP medium (all mediums are provided by Chengdu Litian Century Technology Co., L...

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Abstract

The invention discloses three-flavor lactic acid bacteria for ensiling of an alpine postoral area, wherein the optimal growth temperature is 15 DEG C, and the lactic acid bacteria has the characteristics of high growth speed, large acid production amount and the like and provides favorable conditions for the blending of ensiling. The lactic acid bacteria can be rapidly fermented (shorter than or equal to 20d) at low temperature, the contents of lactic acid and total acids are increased, the pH value, the ammoniacal nitrogen / total nitrogen ratio and the contents of propionic acid and butyric acid are reduced, the fermentation quality of an ensiling feed is improved, the crude protein content of the ensiling feed and in vitro dry matter digestion rate are increased, and the lactic acid bacteria can be used as an additive in the ensiling blending process.

Description

Technical field [0001] The invention belongs to the field of animal husbandry, and specifically relates to 3 strains of lactic acid bacteria used for silage in alpine pastoral areas. Background technique [0002] With the development of animal husbandry, lactic acid bacteria preparations are more and more widely used in silage. A large number of studies have shown that the effect of adding lactic acid bacteria varies due to the type of metabolism, raw material characteristics and climate environment. The number and composition of lactic acid bacteria attached to forages in cold regions are different from temperate regions and tropical regions. The commercial lactic acid bacteria currently in use are mainly derived from temperate environments. At the same time, some studies aim to screen the dominant lactic acid bacteria that adapt to silage preparation in tropical regions. [0003] However, there are very few researches on the physiological and biochemical characteristics and app...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K30/18C12R1/25C12R1/225C12R1/01
Inventor 李平白史且沈益新李达旭游明鸿张玉苟文龙
Owner SICHUAN ACAD OF GRASSLAND SCI
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