Composition with ZJ10520 and biogenic bactericide
A ZJ10520, fungicide technology, applied in the field of pesticides, can solve the problems of reducing the control effect, the crops produce bacteria resistance, etc., and achieve the effect of reducing the dosage, excellent synergistic effect, and good sterilization effect.
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Embodiment 1
[0063] Example 1, ZJ10520 combined with Jinggangmycin for indoor toxicity determination of rice blight
[0064] Test object: Rice blight (Rhizoctonia solani Kühn), provided by the strain room of the unit.
[0065] Test method: refer to the method of mycelial growth rate in "Agricultural Industry Standard of the People's Republic of China NY / T 1156.2-2006". Each agent was treated with 5 doses according to the content of the active ingredients. The above-mentioned pathogenic bacteria were cultivated with PDA medium. The bacteria block was moved to the center of the pre-prepared toxic PDA medium, and then cultured in a 25°C incubator, and each treatment was repeated 4 times. Depending on the growth of the CK colony, use the cross method to measure the colony diameter cm of each treatment with a caliper, and calculate the corrected inhibition percentage. Through the linear regression analysis between the probability value of the inhibition rate and the logarithmic value of the s...
Embodiment 2
[0077] Example 2, Indoor Toxicity Determination of ZJ10520 and Ningnanmycin Compound to Rice Smut Disease
[0078] Test object: rice smut fungus (Ustilaginoidea virens), which is preserved by our laboratory and has been identified as having strong pathogenicity. The test method and drug efficacy calculation refer to Example 1.
[0079] Table 2. Indoor toxicity test results of ZJ10520 and Ningnanmycin compound against rice false smut
[0080] potion
Embodiment 3
[0081] Example 3, Determination of indoor activity of ZJ10520 compounded with polyoxin against wheat powdery mildew
[0082] Test object: wheat powdery mildew (Erysiphe graminis), subcultured by long-term living organisms in our laboratory.
[0083] Test method: select a susceptible variety (Yangmai No. 6) for pot planting, wait for the seedlings to grow to the 2-3 leaf stage, spray and dry naturally, shake off the fresh spores of powdery mildew bacteria produced within 24 hours on the diseased wheat leaves Inoculate on potted wheat seedlings at the 2-3 leaf stage treated with the medicament. After inoculation, the test materials were moved to 20°C and controlled relative humidity of about 80% for cultivation in a greenhouse glass room. Pay attention to keeping the pot soil moist every day, and investigate according to the incidence of the blank control in about 7 days. Refer to Example 1 for the calculation of the co-toxicity coefficient.
[0084] Grading standards:
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