Platelet enrichment fluid

A technology of enriching liquid and platelets, which is applied in the field of medical clinical plastic surgery, can solve the problems of no obvious effect, and achieve the effect of easy operation and avoiding waste

Inactive Publication Date: 2018-01-23
安徽信灵检验医学科技股份有限公司
View PDF5 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But this single blood preservation solution can only anticoagulate blood and maintain the basic shape of blood cells
However, for the biggest problem in the current extraction of platelet-rich plasma (PRP): increasing the concentration of platelets in the prepared platelet-rich plasma, simplifying the operation steps, and reducing pollution have no obvious effect.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Platelet enrichment fluid
  • Platelet enrichment fluid
  • Platelet enrichment fluid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0027] A platelet-enriched solution, consisting of the following raw materials in parts by weight (%):

[0028]

[0029]

[0030] Preparation method: In addition to water, the above-mentioned raw materials are weighed in conical flasks by weight, and finally, normal temperature distilled water is weighed. Shake the Erlenmeyer flask to dissolve all the reagents in it, and then let it stand for 20 minutes. Then use a Buchner funnel with a filter membrane pore size of 0.1um to suction filter into the reagent bottle, add the filtered platelet enrichment solution to the blood collection tube according to the amount of 1ml platelet enrichment solution to 7ml blood, and pump the blood collection tube into a suitable vacuum Afterwards, steam sterilize for later use.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses platelet enrichment fluid. The platelet enrichment fluid is prepared from the following raw materials in parts by weight: 2 to 2.5 % of sodium citrate, 0.6 to 1 part of citricacid, 2.1 to 2.5 % of glucose, 2.5 to 3.5 % of hydroxyethyl starch 200 / 0.5, 2.5 to 3.5 % of dextran 70 and 87 to 90.3 % of distilled water. The platelet enrichment fluid can change the specific gravity of centrifuged plasma to 1.04 to 1.05 and the osmotic pressure of the enrichment fluid is isotonic to hemocyte. Therefore, after one-time centrifuging, because the specific gravity of the platelet in a blood-collection tube is smaller than that of the plasma, the platelet is enriched on the upper layer of the plasma, and the loss of the platelet caused by metastasis is prevented; in addition, according to the method, operation procedures are greatly simplified, thereby reducing pollution.

Description

technical field [0001] The invention belongs to the field of medical clinical plastic surgery, and relates to a blood collection tube additive for increasing the concentration of platelets in prepared platelet-rich plasma (PRP), in particular to a platelet enrichment solution. Background technique [0002] Platelet-rich plasma (PRP) currently used in the field of medical clinical plastic surgery is mostly obtained by secondary centrifugation using the difference in the proportion of each component in the blood. The specific gravity of blood cells in blood is 1.07-1.10, the specific gravity of platelets is 1.030-1.04, and the specific gravity of plasma is 1.025-1.030. After centrifugation, platelets will be in the lower layer of plasma. When preparing PRP, the autologous blood is first collected in blood collection tubes added with blood preservation solution (ACD solution), and then all the supernatant is taken out by one centrifugation immediately and transferred to another...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): B01D17/05B01D17/038C09K3/00G01N1/34G01N15/10
Inventor 金黄根王之侃胡圆圆吴向阳
Owner 安徽信灵检验医学科技股份有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products