Complex probiotic preparation capable of improving intestinal function of chick and preparation method thereof
A technology for compounding probiotics and intestinal functions, applied in biochemical equipment and methods, microorganism-based methods, bacteria, etc., can solve the problems of low product quality, threat to human health, and many diseases of livestock and poultry, and improve the intestinal tract. functional, immune-enhancing and growth-enhancing effects
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[0023] 1. Materials
[0024] The methods used in this example are conventional methods known to those skilled in the art unless otherwise specified, and the reagents and other materials used are all commercially available products unless otherwise specified.
[0025] 2. Method
[0026] 2.1 Preparation of mixed bacterial solution
[0027] Weigh 25 grams of LB medium, dissolve it in 1000m L of tap water, mix well, divide it into three parts, sterilize with high pressure steam at 121°C for 20 minutes, and inoculate 5 mL of Bacillus velesii, Enterococcus and oligotrophic monosporum maltophilia Bacteria seed bacterial liquid, under the condition of 37 ℃, 120r / min, carry out expansion culture, adjust to the concentration of Bacillus Veles, Enterococcus, Stenotrophomonas maltophilia respectively 5.5-6.5×10 13 CFU / mL, 3.5-4.5×10 12 CFU / mL, 5.5-6.5×10 12 CFU / ml.
[0028] The formula of LB medium is: tryptone 10g, yeast extract 5g, NaCl 10g.
[0029] The expanded cultured Bacillus...
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