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Method for preventing selfing by performing artificial emasculation on witloof and increasing cross-pollination hybridizing rate of witloof

A technology for artificial emasculation and cross-pollination, which is applied in the fields of botanical equipment and methods, applications, and plant genetic improvement, can solve the problems of low hybridization rate, low breeding success rate, troublesome operation, etc., and achieves simple operation method, The effect of improving the cross-pollination hybridization rate and the success rate

Active Publication Date: 2018-02-23
华毓农业开发(烟台市)有限责任公司
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AI Technical Summary

Problems solved by technology

[0002] The existing chicory pollination method has low hybridization rate, troublesome operation, and low breeding success rate, so how to improve its hybridization rate and breeding success rate has become an important topic in this field

Method used

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Embodiment Construction

[0020] A kind of artificial detasseling of chicory prevents selfing and improves the method for its cross-pollination hybridization rate, comprises the following steps:

[0021] (1) Cultivation of hybrid male and female plants

[0022] Adopt field cultivation conditions and management routine methods suitable for the normal growth of chicory to ensure that the hybrid male and female plants grow normally and develop robustly, and ensure that the flowering periods of the two are basically the same, and cultivate chicory so that the hybrid male and female plants grow normally, The growth is strong, and the flowering period of the two is basically the same. Select the target traits, healthy and disease-free parental plants as hybrid plants, and select robust flower branches and flower buds before hybridization; and perform appropriate thinning of flowers and branches.

[0023] (2) Preparation of pollen

[0024] In mid-June, chicory enters its full flowering period. Two or three d...

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Abstract

The invention discloses a method for preventing selfing by performing artificial emasculation on witloof and increasing the cross-pollination hybridizing rate of the witloof. The witloof is in a full-bloom stage in the middle of June, inflorescence which is healthy, does not have plant diseases and insect pests and has anther not bloomed is selected at about 8 o'clock in the morning of cloudless and windless weather, a pointed tweezers move from bottom to top along the gap between filaments of a flower base, the gathered anther walls are torn, pollen is not touched in the operating process, the anther at the top of a stamen is removed, the anther clings to a stylet, the stylet is washed by a clean water-containing small bottle with a spraying device to prevent selfing, a pencil with an erasehead is used for dipping the pollen of different varieties of witloof to coat the stylet head, and the whole process of cross-pollination is completed. The operating method is simple, damage to petal and pistil is avoided, the selfing maturing rate of the witloof can be effectively reduced, the cross-pollination hybridizing rate of the witloof is obviously increased and the success rate of artificial cross breeding is increased.

Description

technical field [0001] The invention relates to a method for cross-pollination of chicory. Background technique [0002] The existing chicory pollination method has low hybridization rate, troublesome operation and low breeding success rate, so how to improve its hybridization rate and breeding success rate has become an important topic in this field. Contents of the invention [0003] The object of the present invention is to provide a method for artificial detasseling of chicory with high hybridization rate to prevent self-fertilization and improve its cross-pollination hybridization rate. [0004] Technical solution of the present invention is: [0005] A method for artificial detasseling of chicory to prevent self-fertilization and improve its cross-pollination hybridization rate is characterized in that it comprises the following steps: [0006] (1) Cultivation of hybrid male and female plants [0007] Cultivate chicory so that the hybrid male and female plants gro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02
CPCA01H1/02
Inventor 梁美霞
Owner 华毓农业开发(烟台市)有限责任公司
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