Biological preparation containing liver stem cells and preparation method of biological preparation

A technology for biological preparations and hepatic stem cells, which is applied to medical preparations, drug combinations, and pharmaceutical formulations containing active ingredients, can solve problems such as the lack of application of hepatic stem cells, and achieve the goal of ensuring stem cell activity, good therapeutic effect, and simple preparation process. Effect

Inactive Publication Date: 2018-03-06
金时代进出口贸易重庆有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently, there is a lack of application of hepatic stem cells in biological preparations for liver injury. How to create a biological preparation containing hepatic stem cells has become an urgent problem to be solved by those skilled in the art.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A biological preparation containing hepatic stem cells, its raw materials are as follows by weight: 5ml of hepatic stem cells, 2ml of peripheral blood stem cells, 3ml of albumin, 2 grams of glucose, 2ml of 50% ethanol, 1 gram of vitamin A, 100ml of solvent, the hepatic stem cells The content is 5×10 5 ~2×10 8 individual / ml, the content of the peripheral blood stem cells is 4×10 5 ~1×10 8 pieces / ml, the content of the albumin is 1-30wt%, and the solvent is 0.9% sodium chloride aqueous solution.

[0022] A method for preparing a biological preparation containing liver stem cells, comprising the steps of:

[0023] S1: Weigh the liver stem cells, peripheral blood stem cells, albumin, glucose 2-4 grams, 50% ethanol, vitamin A 1-1.2 and solvent in proportion for later use;

[0024] S2: Take one-third of the hepatic stem cell solution, one-third of the glucose, one-third of the ethanol solution, one-third of the vitamin A and one-third of the solvent, and put them into a m...

Embodiment 2

[0030] A kind of biological preparation containing hepatic stem cells, its raw materials are as follows by weight: 6ml of hepatic stem cells, 3ml of peripheral blood stem cells, 4ml of albumin, 3 grams of glucose, 2.5ml of 50% ethanol, 1.1 grams of vitamin A, 110ml of solvent, the liver The content of stem cells is 5 x 10 5 ~2×10 8 individual / ml, the content of the peripheral blood stem cells is 4×10 5 ~1×10 8 pieces / ml, the content of the albumin is 1-30wt%, and the solvent is 0.9% sodium chloride aqueous solution.

[0031] A method for preparing a biological preparation containing liver stem cells, comprising the steps of:

[0032] S1: Weigh the liver stem cells, peripheral blood stem cells, albumin, glucose 2-4 grams, 50% ethanol, vitamin A 1-1.2 and solvent in proportion for later use;

[0033] S2: Take one-third of the hepatic stem cell solution, one-third of the glucose, one-third of the ethanol solution, one-third of the vitamin A and one-third of the solvent, and p...

Embodiment 3

[0039] A biological preparation containing hepatic stem cells, its raw materials are as follows by weight: 8ml of hepatic stem cells, 4ml of peripheral blood stem cells, 5ml of albumin, 4 grams of glucose, 3ml of 50% ethanol, 1.2 grams of vitamin A, 120ml of solvent, the hepatic stem cells The content is 5×10 5 ~2×10 8 individual / ml, the content of the peripheral blood stem cells is 4×10 5 ~1×10 8 pieces / ml, the content of the albumin is 1-30wt%, and the solvent is 0.9% sodium chloride aqueous solution.

[0040] A method for preparing a biological preparation containing liver stem cells, comprising the steps of:

[0041] S1: Weigh the liver stem cells, peripheral blood stem cells, albumin, glucose 2-4 grams, 50% ethanol, vitamin A 1-1.2 and solvent in proportion for later use;

[0042] S2: Take one-third of the hepatic stem cell solution, one-third of the glucose, one-third of the ethanol solution, one-third of the vitamin A and one-third of the solvent, and put them into ...

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PUM

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Abstract

The invention discloses a biological preparation containing liver stem cells. The biological preparation contains the following raw materials in parts by weight: 5ml-7ml of the liver stem cells, 2ml-4ml of peripheral blood stem cells, 3ml-5ml of albumin, 2g-4g of glucose, 2ml-3ml of 50% ethanol, 1g-1.2g of vitamin A and 100ml-120ml of a solvent. The solvent is a 0.9% sodium chloride; stem cells are primitive cells for forming each tissues and organs of mammals, cell nucleuses are mostly euchromatins and have relatively high telomerase activity, the stem cells can be divided into embryonic stemcells and adult stem cells, and the liver stem cells, the peripheral blood stem cells and the albumin are combined to be prepared into a stem cell biological preparation, so that the tissue regeneration of injured parts of livers can be effectively promoted, and a relatively good liver injury treatment effect can be achieved; and a preparation process is simple, and a water-bath heating manner isadopted, so that the water temperature can be controlled, the temperature environment of the preparation is guaranteed, the biological preparation has relatively stable performance, and the activityof the stem cells is guaranteed.

Description

technical field [0001] The invention relates to the technical field of biopharmaceuticals, in particular to a method for preparing a biological preparation containing liver stem cells. Background technique [0002] Under the planned economic system, the medical and health system has a clear positioning. China's medical and health has created a series of brilliance, and has made great achievements in various aspects such as medical services and preventive health care. Medical services in rural and urban areas were also in full swing at this time, and the accessibility of medical services was greatly enhanced. Due to the influence of social and economic development and comprehensive national strength, as well as the management of "political and political integration", China has certain problems in medical technology, service level and infrastructure construction to varying degrees. This requires people to constantly explore new ways of development. China's medical security s...

Claims

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Application Information

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IPC IPC(8): A61K38/38A61K35/407A61K35/14A61P1/16
CPCA61K35/14A61K35/407A61K38/38A61K2300/00
Inventor 熊华强胡小东宋彩霞黄启程王仁杰邱阳唐玲李国炎
Owner 金时代进出口贸易重庆有限公司
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