Tangshen root in-vitro regeneration system establishment technique

A technology of in vitro regeneration and Codonopsis pilosula, which is applied in the field of plant cultivation, can solve the problems that wild Codonopsis pilosula resources cannot adapt to large-scale and standardized production, hinder the development of Codonopsis pilosula industry, and soil desertification, and achieve pure varieties, convenient operation, and robust seedlings Effect

Inactive Publication Date: 2018-03-16
陈培党
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional method of obtaining Codonopsis pilosula is to dig wild plant resources, causing soil erosion and severe soil desertification
In addition, Codonopsis pilosula seeds mainly come from the wild, and its variet

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] (1) Treatment of explants: Select robust Codonopsis pilosula plants and cut stems with axillary buds as explants, cut off the leaves, cut into 5.2-long segments with 4 axillary buds, and wash them with washing powder water for 3 hours. Then use a soft brush to gently scrub the surface of the explant to remove the dust and some bacteria on the surface, then rinse it with tap water for 1 hour, then place it in an ultra-clean workbench, first disinfect it with 75% ethanol for 8 seconds, and then wash it 4 times with sterile water , and then sterilized with 0.1% mercuric chloride solution for 6 minutes, rinsed with sterile water for 6 times, and then dried the water droplets on the surface with sterile filter paper before use.

[0017] (2) Clumping bud guidance: Cut the stems of Codonopsis pilosula with axillary buds treated in step (1) into 3.0cm pieces and inoculate them on the induction medium, culture them in total darkness at 29°C for 5 days, and then light them for 13 ...

Embodiment 2

[0022] (1) Treatment of explants: Select robust Codonopsis pilosula plants and cut stems with axillary buds as explants, cut off the leaves, cut into 5.4 long segments with 5 axillary buds, and wash them with washing powder water for 6 hours. Then use a soft brush to gently scrub the surface of the explant to remove the dust and some bacteria on the surface, then rinse it with tap water for 5 hours, then place it in an ultra-clean workbench, first disinfect it with 75% ethanol for 14 seconds, and then wash it 7 times with sterile water , and then sterilized with 0.1% mercuric chloride solution for 10 minutes, rinsed with sterile water for 8 times, and then dried the water droplets on the surface with sterile filter paper before use.

[0023] (2) Tufted bud guide: cut the stems with axillary buds treated in step (1) into 4.25cm pieces and inoculate them on the induction medium, culture them in total darkness at 29°C for 1 day, and then light them for 15 hours a day. The light i...

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Abstract

The invention discloses a tangshen root in-vitro regeneration system establishment technique. Tangshen roots are platycodon grandiflorum plants and are dry tangshen roots. The tangshen roots are dug in autumn, separated according to thicknesses, repeatedly rubbed and dried in the sun after mud removal, and finally sun-dried. Main components comprise codonopsis pilosula polysaccharides, tangshenoside I, codonopsine, taraxeryl acetate, eleven essential microelements, various amino acids and synanthrin. The tangshen roots have the functions of tonifying middle-Jiao and Qi and strengthening the spleen and stomach, and are used for patients suffering from spleen deficiency, food deficiency, loose stool, weakness of limbs, palpitation, breath shortness, anal prolapse and uterine prolapse. A stemwith axillary buds serves as an explant, tissue culture and rapid propagation of the tangshen roots are realized by processes of multiple-shoot inducement, propagation culture, root and shoot inducement, test-tube seedling domestication and transplanting and the like, and technical guarantee is provided for industrial development of the tangshen roots. The technique is easy to master and convenient to operate, obtained seed seedlings are healthy and strong, rapid in growth and germination, pure in variety and high in germination rate, and market requirements are met.

Description

technical field [0001] The invention belongs to the category of plant cultivation, and in particular relates to a technology for establishing an in vitro regeneration system of Codonopsis pilosula. Background technique [0002] Codonopsis Codonopsis is the plant of Platycodonaceae, the dry root of Codonopsis pilosula. It is excavated in autumn, separated according to thickness, removed from mud, rubbed and dried repeatedly, and finally dried in the sun. Codonopsis root is cylindrical, with thin ends and some branches, 10-33cm long and 0.7-2.8cm in diameter. The surface is yellow-gray, with "lion pan head" on the root head, and the top of the stem scar is concave. The wild product has a large root head and many stem scars, and the cultivated product has a small root scar; there are ring-shaped horizontal stripes under the root head, and the wild product has more. Dense, with few or no cultivars; the whole has longitudinal stripes and scattered horizontal and long lenticels;...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 陈培党
Owner 陈培党
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