Tissue culture and rapid propagation method of Hydrocotyle sibthorpioides
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A tissue culture and coriander technology, applied in the field of plant tissue culture, can solve the problems of scarcity of seeds, soil desertification, mixed varieties, etc., and achieve the effects of pure seedling varieties, fast growth and germination, and robust seedlings.
Inactive Publication Date: 2018-02-13
陈金水
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Problems solved by technology
The traditional method of obtaining coriander is to dig wild plant resources in the wild, which is easy to cause soil erosion and severe soil desertification
In addition, the seeds of coriander are mainly from the wild, the varieties are mixed, the seeds are scarce, and the germination is extremely low. The resources of wild coriander can no longer meet the needs of large-scale and standardized production, which has become the biggest bottleneck hindering the development of coriander industry.
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Embodiment 1
[0016] (1) Treatment of explants: Select the stem segment with axillary buds from the strong coriander plant as an explant, cut off the leaves, cut into 6 cm long segments with 3 axillary buds, and wash them with washing powder water first. Rinse for 4 hours, then gently scrub the surface of the explants with a soft brush to remove the dust and some bacteria on the surface, then rinse with tap water for 1 hour, then place it in an ultra-clean workbench, first sterilize it with 75% ethanol for 5 seconds, and then use a sterile Wash 5 times with water, then sterilize with 0.1% mercury liter solution for 7 minutes, rinse 6 times with sterile water, and then dry the water droplets on the surface with sterile filter paper before use.
[0017] (2) Tufted bud guide: cut the stems with axillary buds treated in step (1) into 3.5 cm pieces and inoculate them on the induction medium, culture them in total darkness at 36°C for 5 days, and then place them under light for 13 hour, light int...
Embodiment 2
[0022] (1) Treatment of explants: Select the stem segment with axillary buds from the strong coriander plant as an explant, cut off the leaves, cut into 7 cm long segments with 4 axillary buds, and wash them with washing powder water first. Rinse for 6 hours, then gently scrub the surface of the explants with a soft brush to remove the dust and some bacteria on the surface, then rinse with tap water for 4 hours, then place it in an ultra-clean workbench, first disinfect it with 75% ethanol for 12 seconds, and then use a sterile Wash 7 times with water, then disinfect with 0.1% mercuric chloride solution for 9 minutes, rinse 7 times with sterile water, and then dry the water droplets on the surface with sterile filter paper before use.
[0023] (2) Tufted bud guide: cut the stems with axillary buds treated in step (1) into 3.5 cm pieces and inoculate them on the induction medium, culture them in total darkness at 30°C for 6 days, and then place them under light for 15 hour, lig...
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Abstract
The invention discloses a tissue culture and rapid propagation method of Hydrocotyle sibthorpioides; Hydrocotyle sibthorpioides is a perennial herb, the stem is slime and fragile and crawls, and the herb spreads on the ground in sheeting form, bared or nearly bared, the nodal segment bears root, the alternate simple leaves are round or nearly kidney shaped, the base is cordate, lobes are short, with blunt teeth, the upper surface of the leaf is dark green, and the lower surface is green; the petiole is slim, with opposite umbel and leaves simply grown on the segment, and the bract is oblanceolate with umbel. All-grass of Hydrocotyle sibthorpioides is collected in summer and fall and is dried in sunlight. Hydrocotyle sibthorpioides grows in wet soil of south China. Stems of Hydrocotyle sibthorpioides with auxiliary bud are used as explants to establish an in-vitro rapid propagation system of Hydrocotyle sibthorpioides, so that basis is laid for large-scale production of Hydrocotyle sibthorpioides. The stems with auxiliary bud are used as explants to provide tissue culture and rapid propagation of Hydrocotyle sibthorpioides via the processes such as budding induction, enrichment culture, root-seedling induction and test-tube seedling domestication and transplanting, and technical support is provided for the industrial development of Hydrocotyle sibthorpioides.
Description
technical field [0001] The invention relates to a method for plant tissue culture in agricultural biotechnology, in particular to a method for rapidly propagating tissue culture of coriander. Background technique [0002] Sky coriander is also known as: chicken intestines, broken copper coins, wild celery, and ground grass. Perennial herb. The stems are thin and long, creeping, spread flat on the ground in patches, bald or nearly bald; rooting on the stem nodes. Alternate single leaves, round or nearly kidney-shaped, heart-shaped at the base, short lobes, cognate, dark green above, green below; petioles are slender, umbels and leaves are opposite, solitary on nodes, bracts oblanceolate Shaped, umbel. Flowers from April to May, green and white, with sessile or stalked flowers; calyx teeth lacking, petals are ovate, arranged in valvate shape, double-hanging fruits are slightly heart-shaped, the sides of the meric fruits are flat, smooth or spotted, and the dorsal ribs Slig...
Claims
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Application Information
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