Use of deoxyribonucleic acid in promotion of protein crystallization and method for promoting protein crystallization

A deoxyribonucleic acid and protein crystallization technology, which is applied in the field of deoxyribonucleic acid to promote protein crystallization and promote protein crystallization, to achieve the effects of improving success rate and efficiency, eliminating interference or polluting particles, and conveniently obtaining materials

Inactive Publication Date: 2018-03-20
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] However, methods to facilitate protein cryst...

Method used

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  • Use of deoxyribonucleic acid in promotion of protein crystallization and method for promoting protein crystallization
  • Use of deoxyribonucleic acid in promotion of protein crystallization and method for promoting protein crystallization
  • Use of deoxyribonucleic acid in promotion of protein crystallization and method for promoting protein crystallization

Examples

Experimental program
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Embodiment 1

[0035] First, precipitants containing different concentrations of herring sperm DNA were prepared, and the DNA concentrations were 0, 10, 20, 30, 40, and 50 mg / mL. Prepare protein solutions with different concentrations, and the protein in this application is a model protein-lysozyme (Lysozyme). The concentration of protein solution is 2.5, 5.0, 7.5, 10.0mg / mL. All solutions were filtered through a 0.02 μM filter. Then use the hanging drop method and equal volume mixing. After mixing, the DNA concentration is 0, 5, 10, 15, 20, 25mg / mL, and the protein concentration is 1.25, 2.5, 3.75, 5mg / mL. Crystallize proteins at different concentrations such as figure 1 shown. Different concentrations of DNA were used to promote the crystallization of protein solutions with different concentrations, and each experiment was repeated 48 times. Observe with an optical microscope every day, take pictures to record the crystal growth and count the success rate of crystallization.

[0036] ...

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Abstract

The present invention discloses use of deoxyribonucleic acid in promoting of protein crystallization. The deoxyribonucleic acid can significantly increase the efficiency and success rate of the protein crystallization.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular, the invention relates to the use of deoxyribonucleic acid in promoting protein crystallization and the method for promoting protein crystallization. Background technique [0002] Protein is an important part of organisms, and analyzing the crystal structure of protein is beneficial for people to study a series of life characteristics and physiological mechanisms. The method of studying protein crystallization is of great significance to the cognition of living organisms and protein purification. However, protein crystallization is sensitive to many factors such as temperature, pH, salt concentration, polymers, organic matter, etc. The research on protein crystallization methods is mostly based on previous experience. Different methods have been explored for facilitating protein crystallization. Such as modifying different surfaces, and introducing different methods such as magnetic f...

Claims

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Application Information

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IPC IPC(8): C07K1/30
CPCC07K1/306
Inventor 杨忠强张波
Owner TSINGHUA UNIV
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