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Cell slide glass specimen production apparatus, cell slide glass specimen production method, and DNA or RNA extraction method

A technique for making specimens and glass slides, which is applied in the fields of DNA preparation, recombinant DNA technology, and methods for sampling biological materials, and can solve the problems of undisclosed CTC slide specimen devices and methods.

Inactive Publication Date: 2018-04-03
OPTNICS PRECISION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, up to now, no related device and method for making CTC slide specimens have been disclosed

Method used

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  • Cell slide glass specimen production apparatus, cell slide glass specimen production method, and DNA or RNA extraction method
  • Cell slide glass specimen production apparatus, cell slide glass specimen production method, and DNA or RNA extraction method
  • Cell slide glass specimen production apparatus, cell slide glass specimen production method, and DNA or RNA extraction method

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Experimental program
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no. 1 approach

[0046] In the present embodiment, "circulating cancer cells" refer to circulating tumor cells contained in the blood of cancer patients, and are rare cells with a very small number of cells usually present in 1 cc of blood. Circulating cancer cells take the initials of the English translation "Circulating Tumor Cell", also known as "CTC".

[0047] "Rare cancer cells" refers to rare tumor cells contained in body fluids such as ascites, peritoneal washings, lymph, and bone marrow fluid of cancer patients.

[0048] First, in figure 1 Herein, the filter 16 used in this embodiment and the filter unit 28 to which the filter 16 is attached will be described.

[0049] (filter)

[0050] The filter 16 is to form a very large number of holes 26 evenly or regularly on an extremely thin plate (the hole density is 1×10 4 / cm 2 above) to get the filter. The density of the holes 26 varies according to the arrangement forms such as lattice arrangement and zigzag arrangement, and is usual...

no. 2 approach

[0093] exist Figure 17 Among them, the cell slide sample preparation method described in this embodiment has the same first step S1 as that of the first embodiment (see also figure 1 ), and the second step S12 to the fourth step S14. In the drawings, the same reference numerals are assigned to the same parts as those of the first embodiment, and description thereof will be omitted.

[0094] In the first step S1 , the target cells 12 are not fixed and treated with surfactants, and the target cells 12 are kept alive and directly enter the second step S12 .

[0095] In the second step S12, the filter 16 taken out from the filter unit 28 is placed on the glass slide 42 coated with adhesion-promoting substances such as type I collagen, and then the culture solution 58 is immediately added to make the target cells 12 such as Adhesion occurs under survivable conditions.

[0096] In the third step S13, by placing the target cell 12 in CO 2 The incubator is heated at 37° C. for 1...

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Abstract

A cell slide glass specimen production apparatus is provided with: a filter comprising a concavity that traps target cells, i.e., circulating cancer cells in the blood and / or rare cancer cells in bodily fluids, and holes that are formed in the concavity and allow non-target cells to pass therethrough; a slide glass that is laid over the target-cell-trapping surface of the filter so that the targetcells are transferred thereto; a cover member that covers the surface of the filter on the side opposite the slide glass and seals in a physiological buffer solution for immersing the target cells within the concavity; and a container that stores a preservative solution into which the slide glass is immersed, preserving the target cells transferred onto the slide glass.

Description

technical field [0001] The present invention relates to a device for preparing a slide specimen of target cells (circulating cancer cells in blood or rare cancer cells in body fluid), a method for preparing a slide specimen of cells, and a method for extracting DNA or RNA. Background technique [0002] Various devices have been reported as devices for separating and detecting circulating tumor cells (Circulating Tumor Cells; hereinafter also referred to as "CTC") in blood, but CellSearch (registered trademark) of Veridex Corporation is a U.S. Food and Drug Administration (FDA) the only device approved and marketed. [0003] In addition, Japanese Patent Application Laid-Open No. 6-221976 and U.S. Patent No. 5,143,627 disclose that general cells can be easily and efficiently transferred to a glass slide using a filter when processing a general cell diagnostic sample such as sputum, and the slide is prepared. Apparatus and methods for slide specimens. However, up to now, no r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/34C12M1/00C12M1/26G01N1/28G02B21/34
CPCC12N15/1003G01N1/2813G01N1/312G01N1/4077G01N2001/4088C12Q1/6806G02B21/34C12M1/00C12M1/26C12M1/34G01N1/28
Inventor 绢田精镇市野泽义行中西速夫伊藤诚二
Owner OPTNICS PRECISION
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