Method for inducing calluses of hemp seeds

A technology of callus and hemp, which is applied in the field of hemp seed induction callus, can solve the problems that hinder the large-scale production of hemp, cannot meet the demand of hemp, and the low germination rate of seeds, so as to improve the sterilization effect, The effect of increasing germination rate and reducing pollution rate

Active Publication Date: 2018-04-17
BAISE UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the breeding technology of hemp is mainly based on seed propagation, that is, sexual reproduction, which has disadvantages such as low seed germination rate, long germin

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0020] Example 1

[0021] A method for inducing callus from hemp seeds, comprising the following steps:

[0022] (1) Pretreatment: Select the hemp seeds that are full and uniform in size, rinse with running water, use ultrasonic waves with a power of 100W and a frequency of 30KHz for 20min, and keep the hemp seeds immersed in the medium for 30min. The medium for the first 10 minutes was water at 40°C, and the medium for 10 minutes after the treatment was GGR solution with a mass concentration of 25 mg / L; the hemp seeds after ultrasonication were placed in a petri dish covered with a damp cloth, and then covered with a layer of damp cloth After that, placed at 25°C for 8h;

[0023] (2) Disinfection treatment: the above-mentioned pretreated hemp seeds are peeled off to obtain hemp seeds, and the hemp seeds are rinsed with running water for 10 min, treated with 70% alcohol by volume for 15 s, and then used Rinse 3 times with sterile water, then treat with 0.1% mercuric chloride...

Example Embodiment

[0025] Example 2

[0026] A method for inducing callus from hemp seeds, comprising the following steps:

[0027] (1) Pretreatment: select the hemp seeds that are full and uniform in size, rinse them with running water, use ultrasonic waves with a power of 110W and a frequency of 30KHz for 20min, and keep the hemp seeds immersed in the medium for 45min. The medium for the first 10 minutes was water at 43°C, and the medium for 10 minutes after the treatment was GGR solution with a mass concentration of 32 mg / L; the hemp seeds after ultrasonic treatment were placed in a Petri dish covered with a damp cloth, and then covered with a layer of damp cloth After that, placed at 25°C for 9h;

[0028] (2) Disinfection treatment: after the above-mentioned pretreated hemp seeds are peeled off the seed coats, hemp seeds are obtained, after the hemp seeds are rinsed with running water for 15 min, treated with alcohol with a volume fraction of 72% for 25 s, and then used Rinse 3 times with ...

Example Embodiment

[0030] Example 3

[0031] A method for inducing callus from hemp seeds, comprising the following steps:

[0032] (1) Pretreatment: select the hemp seeds that are full and uniform in size, rinse them with running water, use ultrasonic waves with a power of 120W and a frequency of 30KHz for 20min, and keep the hemp seeds immersed in the medium for 50min. The medium for the first 10 minutes was water at 45°C, and the medium for 10 minutes after the treatment was GGR solution with a mass concentration of 35 mg / L; the hemp seeds after ultrasonication were placed in a petri dish covered with a damp cloth, and then covered with a layer of damp cloth After that, placed at 25°C for 10h;

[0033] (2) Disinfection treatment: after the above-mentioned pretreated hemp seeds are peeled off the seed coat, hemp seeds are obtained, and after the hemp seeds are rinsed with running water for 20 min, the volume fraction is 75% alcohol for 30 s, and then used Rinse 3 times with sterile water, th...

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Abstract

The invention belongs to the technical field of biology and particularly discloses a method for inducing calluses of hemp seeds. The method comprises the following steps: (1) pretreatment: treating the hemp seeds by virtue of ultrasonic waves; (2) disinfection treatment: treating the hemp seeds by virtue of alcohol and mercury bichloride, and removing seed coats, so as to obtain fructus cannabis;and (3) inoculated culture: inoculating the disinfected fructus cannabis into an induced culture medium, and carrying out culturing, so as to obtain the calluses. According to the method, the callusesare induced through tissue culture by taking the hemp seeds as explants, so that the induction rate is high, the quality of the induced calluses is good, and the foundation is provided for acquiringa large number of healthy and strong tissue culture seedlings through the subsequent cultivation and differentiation of the hemp seeds.

Description

【Technical field】 [0001] The invention relates to the field of biotechnology, in particular to a method for inducing callus with hemp seeds. 【Background technique】 [0002] Hemp, also known as thread hemp and sweat hemp, is an annual herb of the genus Hemp in the Moraceae family. After the hemp seed is peeled, it becomes the hemp seed, which contains a variety of nutrients, such as vitamin B, vitamin E, resin, volatile oil and other components. Hemp seed is rich in oil, its oil content is 30%, higher than that of soybean, unsaturated fatty acid accounts for 70%-80%, mainly linoleic acid, linolenic acid and oleic acid, the content is 0.61% respectively , 24.42%, 52.32%, wherein the ratio of linoleic acid and linolenic acid is close to 2.5, which is the best ratio for human body to absorb fatty acids. Among them, unsaturated fatty acids have anti-oxidation, anti-tumor, and immune-enhancing effects, and can significantly reduce the content of high-density protein serum choles...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01C1/00
CPCA01C1/00A01H4/001A01H4/008
Inventor 农艳丰李健朱德生
Owner BAISE UNIV
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