Method for extracting monetaria moneta Linnaeu acidic polysaccharose with oxidization resisting and liver protecting effects from monetaria moneta Linnaeu

An acidic polysaccharide and anti-oxidation technology, applied in the direction of organic active ingredients, medical preparations containing active ingredients, digestive system, etc., can solve the problem that the specificity of enzyme extraction technology is easily limited, and the separation process of active polysaccharide components is easy to be lost and separated. There are no problems such as orientation in the purification work, so as to achieve the effect of enhancing targetness, expanding new uses, and strengthening liver protection activity

Active Publication Date: 2018-04-24
HUAIYIN INSTITUTE OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Previous scientific research mostly focused on extracting polysaccharides from mussels, mud snails and other shellfish by acid method, alkali method or enzymatic hydrolysis method, but the enzyme extraction technology is easily limited due to the specificity of the enzyme, and the extraction technical conditions More stringent, the decomposed extraction solution is easy to be mixed with other non-target products, and other separation steps are needed to obtain high-purity polysaccharides in the follow-up. Destroy the activity of white shellfish polysaccharide
[0004] In addition, the current main method for polysaccharide activity research is to first extract and purify crude polysaccharides, further separate and obtain each component, and then conduct activity detection; this method has many uncertain factors, and active polysaccharide components are easily lost during the separation process, and extraction and separation The obtained shellfish polysaccharides may not be active, which leads to the lack of orientation in the subsequent separation and purification of each component, and there is a waste of resources such as material resources, manpower, and financial resources.

Method used

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  • Method for extracting monetaria moneta Linnaeu acidic polysaccharose with oxidization resisting and liver protecting effects from monetaria moneta Linnaeu
  • Method for extracting monetaria moneta Linnaeu acidic polysaccharose with oxidization resisting and liver protecting effects from monetaria moneta Linnaeu
  • Method for extracting monetaria moneta Linnaeu acidic polysaccharose with oxidization resisting and liver protecting effects from monetaria moneta Linnaeu

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] a. Clean 5 kg of fresh white shellfish, spit out sand overnight, remove the shell, take the white shellfish meat, put it in an oven at 50°C, dry it, and then pulverize it to obtain 100 g of white shellfish meat powder;

[0034] b. After degreasing and mixing 100g of white shellfish meat powder obtained in step a with 1L of absolute ethanol, put in a water bath for 3 hours at 60°C, and keep stirring, and filter to obtain 80g of filter residue after degreasing; extract the filter residue with 800ml of 60°C boiling water for 2h, filter, and take After the filtrate and the obtained filter residue were repeatedly extracted with boiling water twice, the obtained filtrates were combined and concentrated, and 2L of absolute ethanol was added to the concentrated solution to precipitate, filtered, the precipitate was taken, dissolved in 50ml of water, and freeze-dried to obtain 34.6g of white shell crude polysaccharide ;

[0035] c. Take the white shell crude polysaccharide obtai...

Embodiment 2

[0038] a. Clean 5 kg of fresh white shellfish, spit out sand overnight, remove the shell, take the white shellfish meat, put it in an oven at 55°C, dry it, and then pulverize it to obtain 100 g of white shellfish meat powder;

[0039] b. After degreasing and mixing 100 g of white shellfish meat powder obtained in step a with 1 L of absolute ethanol, put in a water bath for 3.5 hours at 62° C., and keep stirring, and filter to obtain 82 g of filter residue after degreasing; extract the filter residue with 800 ml of boiling water at 62° C. for 3 hours, filter, Take the filtrate, and extract the obtained filter residue with boiling water twice, combine the obtained filtrate and concentrate, add 2L of absolute ethanol to the concentrated solution to precipitate, filter, take the precipitate, dissolve it with 50ml of water, freeze-dry to obtain the white shell crude polysaccharide 35.2 g;

[0040] c. Take the white shell crude polysaccharide obtained in step b, fully dissolve it wi...

Embodiment 3

[0043] a. Clean 5 kg of fresh white shellfish, spit out sand overnight, remove the shell, take the white shellfish meat, put it in a 60°C oven, dry it, and then pulverize it to obtain 100 g of white shellfish meat powder;

[0044] b. After degreasing and mixing 100 g of white shellfish meat powder obtained in step a with 1 L of absolute ethanol, place in a water bath at 65° C. for 4 hours, and continuously stir, and filter to obtain 85 g of filter residue after degreasing; extract the filter residue with 800 ml of 65° C. boiling water for 4 hours, filter, Take the filtrate, and extract the obtained filter residue with boiling water twice, combine the obtained filtrate and concentrate, add 2L of absolute ethanol to the concentrated solution to precipitate, filter, take the precipitate, dissolve it with 50ml of water, and freeze-dry to obtain 36g of white shell crude polysaccharide ;

[0045] c. Take the white shell crude polysaccharide obtained in step b, dissolve it fully with...

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Abstract

The invention discloses a method for extracting monetaria moneta Linnaeu acidic polysaccharose with oxidization resisting and liver protecting effects from monetaria moneta Linnaeu. According to the method, oxidization resistance or liver protection activity is utilized as basis, a water extract and alcohol precipitation method and a Sevag deproteinization method are combined, and the monetaria moneta Linnaeu acidic polysaccharose is extracted from the monetaria moneta Linnaeu for the first time. The method disclosed by the invention has the advantages of simple extracting technology steps, moderate and low-requirement operation conditions, no corrosion to operation equipment, environmental friendliness, no byproduct generation and low production cost, the prepared monetaria moneta Linnaeupolysaccharose has good oxidization resistance and liver protecting activity, and a novel choice is provided for preparing oxidization resisting / liver protecting medicine or health-care products.

Description

technical field [0001] The invention relates to a method for shellfish polysaccharides, in particular to a method for extracting white shellfish acidic polysaccharides with antioxidant and liver-protecting effects from white shellfish. Background technique [0002] Bai Bei ( Monetaria moneta Linnaeus), an edible shellfish, widely exists in the South my country Sea. White shellfish was first recorded in "Shen Nong's Materia Medica", which has the functions of clearing away heat, improving eyesight, diuresis and reducing cloudiness. Modern scientific research has proved that white shellfish is rich in various nutrients, such as polysaccharides, proteins and trace elements. Among them, polysaccharides have many The health care functions of shellfish, such as anti-oxidation, antibacterial, anti-cancer, etc., and most of the biological effects of white shellfish are related to polysaccharides. [0003] At present, there are not many research reports on white shellfish, and the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K31/715A61P1/16
CPCA61K31/715C08B37/0003C08B37/006
Inventor 袁君徐婷婷陈星熊清平景怡许莹王丽萍施徐陶伟莉
Owner HUAIYIN INSTITUTE OF TECHNOLOGY
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