Method for simultaneously detecting various glycopeptides antibiotics in animal derived food
A glycopeptide antibiotic, animal-derived technology, applied in measuring devices, instruments, scientific instruments, etc., to achieve the effect of sensitive, accurate and rapid detection methods and improved recovery rates
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Embodiment 1
[0028] A method for the simultaneous detection of five glycopeptide antibiotics in food of animal origin, among which the five glycopeptide antibiotics telavancin, teicoplanin, oritavancin, dalbavacin, vancomycin Schematic diagram of the structure of vancomycin figure 1 As shown, it mainly includes the following steps:
[0029] Weigh 5g of homogeneous sample, put it in a 50mL polypropylene centrifuge tube, add 20mL 0.1mol / L EDTA-Mcllvaine buffer solution, vortex mix at 1000r / min for 1min, extract on a shaker for 15min, then add 10mL methanol to precipitate protein for 10min , and then use a refrigerated centrifuge to centrifuge at 10,000 r / min for 5 minutes (the temperature is lower than 5° C.), take the supernatant, filter it with fast filter paper, and wait for purification.
[0030] Connect the HLB solid-phase extraction cartridge (60mg, 3mL) and the MCX solid-phase extraction cartridge (60mg, 3mL) in series with an adapter, activate with 3mL of methanol and equilibrate wi...
Embodiment 2-7
[0049] Separately adopt solid-phase extraction columns HLB, C18, MCX, WCX, WAX, MAX to replace the HLB solid-phase extraction column and strong cation (MCX) solid-phase extraction column series extraction in Example 1. The obtained comparative results are as follows Figure 4 shown.
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