Lighting system of plate lighting microscope with variable thickness and length of plate
A lighting system and microscope technology, applied in microscopes, optics, optical components, etc., can solve problems such as changes and inability to apply light sheet fluorescence lighting microscopes, and achieve the effects of eliminating spherical aberration, compact system structure, and reducing system aberrations
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[0040] An illumination system for a light sheet illumination microscope with variable light sheet thickness and length, such as figure 1 As shown, sequentially arranged along the optical axis from the object side to the image side: a laser light source, an afocal zoom beam expander system, a cylindrical lens 5 , and a microscopic objective lens 6 . The wavelength of the laser light source is λ=532nm, and the beam waist radius is 0.5mm. The afocal zoom beam expander system is a four-component mechanically compensated afocal zoom beam expander system of a spherical mirror, and the four-component mechanically compensated afocal zoom beam expander system is a front fixed group 1. Zoom group 2, compensation group 3, and rear fixed group 4. The focal lengths of each component are positive, negative, negative, and positive in turn. The light emitted from the laser source passes through the front fixed group and zoom The group and compensation group are imaged at the front focus of t...
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