The invention discloses a scanning flow type
cell imaging analyzer. The scanning flow type
cell imaging analyzer comprises a liquid flow focusing module, an optical
signal excitation detection module,a scanning
galvanometer, an acquisition and control board card and an upper computer.
Laser is focused into light spots smaller than cells in size,
cell imaging is achieved through
light spot scanning, the
power density of an imaging
light source can be improved, and
fluorescence is effectively excited; when a scanning excitation mode is adopted,
laser full light spots are used for imaging, an overall excitation effect is achieved, and the situation of uneven distribution does not exist; and meanwhile, a problem that the
imaging quality is reduced due to non-uniform excitation of
light spot power caused by unstable liquid flow can be solved. According to the invention, the detection pinhole is formed in the focal plane of the objective lens, so that
stray light outside the focal plane canbe blocked, and
spherical aberration and
chromatic aberration are eliminated; a
photomultiplier is adopted to collect optical signals, and weak signals can be amplified; and compared with a traditional CCD imaging sensitivity, the sensitivity is greatly improved.