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Application of nuclear factor chiB inhibition protein 1 combined with A20 to preparation of medicine for treating fatty livers and related diseases

A technology to inhibit protein and nuclear factor, applied in gene therapy, metabolic disease, drug combination, etc., to achieve high safety effect

Active Publication Date: 2018-05-08
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But there is no report about the function and application of ABIN1 of the present invention in fatty liver and related diseases

Method used

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  • Application of nuclear factor chiB inhibition protein 1 combined with A20 to preparation of medicine for treating fatty livers and related diseases
  • Application of nuclear factor chiB inhibition protein 1 combined with A20 to preparation of medicine for treating fatty livers and related diseases
  • Application of nuclear factor chiB inhibition protein 1 combined with A20 to preparation of medicine for treating fatty livers and related diseases

Examples

Experimental program
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Effect test

Embodiment 1

[0097] [Example 1] Effect of ABIN1 overexpression on fat accumulation in L02 cells

[0098] Construct a lentiviral expression vector that overexpresses ABIN1, transfect HEK-293T cells, package the lentivirus, infect L02 cells to construct a stable cell line that overexpresses ABIN1, and overexpress the empty vector as a control (Con), and detect stable cells by Western blot Whether the strain expresses ABIN1.

[0099] After the detection was completed, the cells were divided into 4 groups, namely: L02 empty control group, ABIN1 overexpressed L02 stable cell line control group, L02-empty experimental group, ABIN1 overexpressed L02 stable cell line experimental group. After the cells adhered to the wall and the cells were cultured to 50% healing, palmitate (PA) and oleic acid (OA) (PA 0.2mM+OA 0.4mM) were added to the two experimental groups for stimulation, and the same amount was added to the control group. After 12 hours, the cell samples of each group were collected and sta...

Embodiment 2

[0101] [Example 2] Effect of overexpression of ABIN1 on fat accumulation in mouse primary liver cells

[0102] Construct a lentiviral expression vector that overexpresses ABIN1, transfect HEK-293T cells, package the lentivirus, and infect mouse primary liver cells to construct a cell model that overexpresses ABIN1, and overexpress the empty vector as a control (Con). Blot was used to detect whether the cells in the ABIN1-infected group overexpressed ABIN1.

[0103] After the detection was completed, the cells were divided into 4 groups, namely: no-load control group, ABIN1 overexpressed cell control group, L02-no-load experimental group, and ABIN1 overexpressed cell experimental group. After the cells adhered to the wall and the cells were plated to a degree of 50% healing, palmitate (PA) and oleic acid (OA) (PA 0.2mM+OA0.4mM) were added to the two experimental groups for stimulation, and the same amount was added to the control group. After 12 hours, the cell samples of each...

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Abstract

The invention discloses application of a nuclear factor chiB inhibition protein 1 combined with A20 to preparation of a medicine for treating fatty livers and related diseases and belongs to novel application of ABIN1 (A20binding Inhibitor of NF-KB Activation). According to the application, an L02 cell line, an ABIN1 over-expression L02 cell line, mouse primary hepatocyte cells and ABIN1 over-expression mouse primary hepatocyte cells are adopted as experiment targets, fatty liver cell models are induced through palmitic acid and oleic acid together, and oil red O dyeing results show that undercombined irritation of the palmitic acid and the oleic acid of a same concentration, the areas of red fat granules in ABIN1 over-expression L02 cells are remarkably reduced when being compared with those of L02 cells. The same oil red O dyeing result trend is also observed in mouse primary hepatocyte cells with over-expressed ABIN1, the result shows that ABIN1 over-expression has a remarkable inhibition function on lipid accumulation in palmitic acid and oleic acid induced fatty liver cells. Therefore, ABIN1 has functions of inhibiting liver lipid accumulation and protecting livers.

Description

technical field [0001] The invention belongs to the field of gene function and application, and particularly relates to a ubiquitin chain binding protein, that is, A20 binding inhibitor of NF-κB activation (ABIN1) binding to A20 in fatty liver and related diseases Function and application, and the application of ABIN1 as a target gene in the preparation of drugs for preventing, alleviating and / or treating fatty liver and related diseases. Background technique [0002] Non-alcoholic fatty liver disease (NAFLD) is a common liver disease, which refers to chronic liver disease characterized by fatty degeneration and lipid accumulation in liver cells in addition to alcohol and other definite liver damage factors. disease. Like high blood pressure and diabetes, fatty liver has become one of the common chronic diseases of contemporary people, and it has become the second most common liver disease after viral hepatitis. The incidence rate is increasing year by year and the age is g...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/17A61K48/00A61P35/00A61P1/16A61P3/04A61P3/06A61P3/10A61P5/50G01N33/68
CPCA61K38/1709A61K48/005G01N33/68G01N2333/435G01N2500/00
Inventor 李红良李枫
Owner WUHAN UNIV
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