Artemisia wannensis extract and its preparation method and use
An extract, the technology of Artemisia persicae, applied in the field of medicine, can solve problems such as no particularly good method, and achieve significant curative effect and wide-ranging effects
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Embodiment 1
[0027] Example 1. Preparation method of Artemisia nigra extract
[0028] Take 500g of dry Artemisia nigra and add water to soak for 0.5-3h and then heat for 0.5-3h, filter to obtain filtrate I, add water to the residue and heat for 0.5-3h to obtain filtrate II, combine filtrate I and II to obtain the aqueous extract of Artemisia nigra . The water extract of Artemisia nigra was sampled on the pretreated macroporous adsorption resin column, and eluted with water and 10%-95% ethanol in sequence. The 10%-95% ethanol eluate is concentrated and freeze-dried to dryness to obtain the Artemisia nigra extract.
Embodiment 2
[0029] Example 2. Determination of total flavonoids in the extract of Artemisia nigra
[0030] Flavonoid standard curve:
[0031] Reagents: rutin, methanol
[0032] Rutin reference substance solution preparation
[0033] Accurately weigh out 5.0mg of rutin, add methanol, dissolve and dilute to 100ml, and get 50μg / ml.
[0034] Pipette the rutin reference solution 0, 1.0ml, 2.0ml, 3.0ml, 4.0ml, 5.0ml, 6.0ml, 7.0ml into a 10ml volumetric flask, add methanol to the mark, shake well, measure the wavelength: 360nm. The results are as follows:
[0035] y=0.1371x+0.0093 R 2 =0.9999
[0036] The preparation of the test product (Artemisia serrata 50% ethanol eluate) solution:
[0037] Take 1ml of the sample solution in an evaporating dish, add 1g of polyamide powder to absorb it, evaporate the solvent in a constant temperature drying oven at 50℃, transfer to a chromatography column, eluting with 30ml petroleum ether, discard the petroleum ether, and use 100ml The total flavonoids are eluted with m...
Embodiment 3
[0042] Example 3. Separation and structural identification of chemical components in the extract of Artemisia annua
[0043] The extract of Artemisia nigra is extracted with dichloromethane, ethyl acetate and other solutions, and the extract is passed through various separation methods such as reversed-phase silica gel column, Sephadex LH-20 gel column, preparative HPLC, ODS low pressure column, etc. Gradient elution with water and other systems, separated 20 flavonoids. Through spectral analysis and literature comparison, the chemical structures are determined to be: 1) 6-methoxy-quercetin-marigold-7-O-β-D-glucoside, 2) quercetin-3-O-β -D glucoside, 3) isorhamnetin, 4) marigold-3-O-β-D-glucoside, 5) acacetin-7-O-β-D-glucoside, 6) rutin , 7) 5,7,4'-trihydroxyflavone, 8) 3,3',4',5,7-pentahydroxyflavone, 9) 5,7,4'-trihydroxy-3'-methoxy Flavonoids, 10) kaempferol, 11) 3,5,7,4'-tetrahydroxy-6-methoxyflavone, 12) 3',4',5,7-tetrahydroxyflavone, 13)5,7, 4'-Trihydroxy-6,3'-Dimethoxyfl...
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