Artemisia wannensis extract and its preparation method and use

An extract, the technology of Artemisia persicae, applied in the field of medicine, can solve problems such as no particularly good method, and achieve significant curative effect and wide-ranging effects

Active Publication Date: 2018-12-25
朴光春
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] There is no particularly good way to treat and prevent liver damage in clinical practice

Method used

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  • Artemisia wannensis extract and its preparation method and use
  • Artemisia wannensis extract and its preparation method and use
  • Artemisia wannensis extract and its preparation method and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1. Preparation method of Artemisia nigra extract

[0028] Take 500g of dry Artemisia nigra and add water to soak for 0.5-3h and then heat for 0.5-3h, filter to obtain filtrate I, add water to the residue and heat for 0.5-3h to obtain filtrate II, combine filtrate I and II to obtain the aqueous extract of Artemisia nigra . The water extract of Artemisia nigra was sampled on the pretreated macroporous adsorption resin column, and eluted with water and 10%-95% ethanol in sequence. The 10%-95% ethanol eluate is concentrated and freeze-dried to dryness to obtain the Artemisia nigra extract.

Embodiment 2

[0029] Example 2. Determination of total flavonoids in the extract of Artemisia nigra

[0030] Flavonoid standard curve:

[0031] Reagents: rutin, methanol

[0032] Rutin reference substance solution preparation

[0033] Accurately weigh out 5.0mg of rutin, add methanol, dissolve and dilute to 100ml, and get 50μg / ml.

[0034] Pipette the rutin reference solution 0, 1.0ml, 2.0ml, 3.0ml, 4.0ml, 5.0ml, 6.0ml, 7.0ml into a 10ml volumetric flask, add methanol to the mark, shake well, measure the wavelength: 360nm. The results are as follows:

[0035] y=0.1371x+0.0093 R 2 =0.9999

[0036] The preparation of the test product (Artemisia serrata 50% ethanol eluate) solution:

[0037] Take 1ml of the sample solution in an evaporating dish, add 1g of polyamide powder to absorb it, evaporate the solvent in a constant temperature drying oven at 50℃, transfer to a chromatography column, eluting with 30ml petroleum ether, discard the petroleum ether, and use 100ml The total flavonoids are eluted with m...

Embodiment 3

[0042] Example 3. Separation and structural identification of chemical components in the extract of Artemisia annua

[0043] The extract of Artemisia nigra is extracted with dichloromethane, ethyl acetate and other solutions, and the extract is passed through various separation methods such as reversed-phase silica gel column, Sephadex LH-20 gel column, preparative HPLC, ODS low pressure column, etc. Gradient elution with water and other systems, separated 20 flavonoids. Through spectral analysis and literature comparison, the chemical structures are determined to be: 1) 6-methoxy-quercetin-marigold-7-O-β-D-glucoside, 2) quercetin-3-O-β -D glucoside, 3) isorhamnetin, 4) marigold-3-O-β-D-glucoside, 5) acacetin-7-O-β-D-glucoside, 6) rutin , 7) 5,7,4'-trihydroxyflavone, 8) 3,3',4',5,7-pentahydroxyflavone, 9) 5,7,4'-trihydroxy-3'-methoxy Flavonoids, 10) kaempferol, 11) 3,5,7,4'-tetrahydroxy-6-methoxyflavone, 12) 3',4',5,7-tetrahydroxyflavone, 13)5,7, 4'-Trihydroxy-6,3'-Dimethoxyfl...

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PUM

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Abstract

The invention discloses an artemisia sacrorum extract which contains total-flavone compounds, wherein the total-flavone compounds comprise one or more of 6-methoxy-quercetagetin-7-O-beta-D-glucoside, quercetin-3-O-beta-D-glucoside, isorhamnetin, queretagetin-3-O-beta-D-glucoside, acacetin-7-O-beta-D-glucoside, rutin, 5,7,4'-trihydroxyflavone, 3,3',4',5,7-pentahydroxyflavone, 5,7,4'-trihydroxy-3'-methoxyflavone, kaempferol, 3,5,7,4'-tetrahydroxy-6-methoxyflavone, 3',4',5,7-tetrahydroxyflavone, 5,7,4'-trihydroxy-6,3'-dimethoxyflavone, 5,7,4'-trihydroxy-6-methoxyflavone, quercitrin, 7,3',4'-trimethyl quercetin, 5-hydroxy-7,4'-dimethoxyflavone, 5,4'-dihydroxy-7-methoxyflavone genkwanin, 5,7-dihydroxy-4'-methoxyflavone, 5,7,3'-dihydroxy-4-methoxyflavone and derivatives. The artemisia sacrorum extract has remarkable prevention and treatment effects on liver injury.

Description

Technical field [0001] The invention relates to the field of medical technology, in particular to an extract of Artemisia nigra and its preparation method and application. Background technique [0002] There are more than 350 species of Artemisia in the genus Compositae, which are widely distributed in temperate regions of the northern hemisphere, Europe, Asia, and North America. A few species are distributed to tropical regions such as Africa, South Asia and Central America. China (excluding the separated Seriphidium( Bess.) Poljak.) has more than 170 species, which are produced everywhere. Among them, A. annua L, A. apiacea Hance, A. capillaris Thunb. and A. argyi Levl. et Vant. are all used as medicine. Artemisia annua can cure malaria and can also make wine cakes. [0003] Artemisia nigra, herb, subshrub or small shrub, often fragrant when rubbed; leaves alternate and often divided; heads are small, heterosexual, disc-shaped, often bent down, arranged in panicles, spikes or ra...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D311/30A61K36/28A61P1/16A61P31/14A61P31/20A61P37/02
CPCA61K36/28A61K2236/33A61K2236/331A61K2236/55
Inventor 朴光春元海丹
Owner 朴光春
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