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Kit for predicting risk of acute myocardial infarction

A technology of acute myocardial infarction and kit, applied in the field of medical diagnosis, can solve the problems of undiscovered miRNAs, etc., achieve good diagnosis and prediction efficiency, and reduce the effect of threats

Inactive Publication Date: 2018-05-08
QINGDAO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

At present, there are few studies on circulating miRNAs in peripheral blood as early biomarkers of AMI, and no relevant reports have been found about miRNAs in urine samples of AMI patients as biomarkers

Method used

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  • Kit for predicting risk of acute myocardial infarction
  • Kit for predicting risk of acute myocardial infarction
  • Kit for predicting risk of acute myocardial infarction

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Experimental program
Comparison scheme
Effect test

Embodiment

[0053] 1. Material and Specimen Collection Description

[0054] AMI patients totaled 32 cases. A total of 19 cases of plasma samples were collected from the normal population. The diagnosis of AMI is confirmed by the currently recognized "gold standard" - cardiac troponin (cTnI) test results. A total of 5ml of blood was collected from each sample using EDTA-Na anticoagulant tubes.

[0055] 2. Sample processing and RNA extraction

[0056] Within 10 minutes after venous blood collection, centrifuge at 3000×g at 25°C for 10 minutes, use RNase-free and bacteria-free tips to take the upper layer of plasma and store it in RNase-free and bacteria-free EP tubes at -80°C for later use . RNA in plasma was extracted and purified by TRIZOL method.

[0057] 3. Real-time fluorescent quantitative PCR (SYBR dye method)

[0058] MicroRNA-22-5p, microRNA-375 and the external reference cel-miR-39 were amplified using the microRNA real-time fluorescent quantitative PCR kit (SYBR PremixEx Ta...

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Abstract

The invention relates to the field of medical diagnosis and in particular to a kit for predicting the risk of acute myocardial infarction. The kit comprises detection reagents of microRNA-22-5p and / ormicroRNA-375. The invention further relates to application of microRNA-22-5p and / or microRNA-375 as molecular markers in preparing diagnosis agents for predicting the risk of acute myocardial infarction. The kit provided by the invention has very good diagnosis and prediction effects on AMI (Acute Myocardial Infarction) and is capable of predicting the disease risk of AMI, instructing preventionand treatment on AMI and alleviating threats of AMI.

Description

technical field [0001] The invention relates to the field of medical diagnosis, in particular to a kit for predicting the risk of acute myocardial infarction. Background technique [0002] (1 Introduction [0003] Cardiovascular disease, also known as circulatory system disease, includes acute rheumatic fever, chronic rheumatic heart disease, hypertensive disease, ischemic heart disease, cor pulmonale (pulmonary heart disease and pulmonary circulation disease), cerebrovascular disease, and Other circulatory system diseases such as heart and blood vessels. Statistics from the World Health Organization in 2015 pointed out that cardiovascular disease is the number one cause of death in the world. In 2012, 17.5 million people died of cardiovascular disease, accounting for 31% of the total global deaths. The number of patients with cardiovascular disease in my country is 290 million, and its morbidity and mortality rank first among various diseases. At present, the risk assess...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883
CPCC12Q1/6883C12Q2600/158C12Q2600/178
Inventor 李培峰王玉丁晗张媛张蕾薛升亓洪昭
Owner QINGDAO UNIV
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