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Tissue culture bottle and tissue culture operation method

A tissue culture and operation method technology, applied in the field of plant tissue culture, can solve the problems of insufficient space height, unsuitable for differentiation and seedling cultivation, etc., to improve efficiency, reduce sterilization and cleaning processes, and improve the integrity and survival of seedlings rate effect

Pending Publication Date: 2018-05-18
上海曼森生物科技有限公司
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  • Abstract
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  • Claims
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Problems solved by technology

The disadvantage of the petri dish is that the height of the space is not enough, it is only suitable for early callus culture, not suitable for later differentiation and seedling culture

Method used

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  • Tissue culture bottle and tissue culture operation method
  • Tissue culture bottle and tissue culture operation method
  • Tissue culture bottle and tissue culture operation method

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Embodiment Construction

[0048] The specific implementation of the tissue culture bottle and the operation method for tissue culture of the present invention will be described in detail below in conjunction with the accompanying drawings.

[0049] Referring to the attached figure, the body of the tissue culture bottle is divided into two sections, which are divided into two parts from the bottom of the bottle body. -5 times. The bottle body 1 provides space for plant growth; the bottle bottom 2 is filled with medium to provide nutrients required for plant growth. When inoculating or transplanting seedlings, remove the body of the bottle and operate directly on the bottom 2 of the bottle, which overcomes the difficulty in operation caused by the small opening and deep depth of traditional tissue culture bottles.

[0050] The bottle body 1 and the bottle bottom 2 are connected by threads or buckles, which is convenient for disassembly and assembly, and prevents falling off. The connection method usual...

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Abstract

The invention relates to the technical field of plant tissue culture, and discloses a tissue culture bottle. The tissue culture bottle comprises a bottle body and a bottle bottom. The bottle body is detachably and hermetically connected with the bottle bottom. An opening is formed in the upper side of the bottle body, and is provided with an air-permeable film. The bottle body is larger than the bottle bottom in height. The invention further discloses a tissue culture operation method by the tissue culture bottle. According to the method, inoculation and plant collection are completed directlyin the bottle bottom or a culture dish. The plant tissue culture and the tissue culture operation method have the advantages that the tissue culture bottle is simple and reasonable in structure and convenient to operate and apply, labor intensity, production cost and pollution are reduced, efficiency of factory-like tissue culture is improved greatly, completeness and survival rate of seedlings are increased, and gains are also increased.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a tissue culture bottle and an operation method for tissue culture. Background technique [0002] Factory plant tissue culture (referred to as tissue culture) has become one of the important manifestations of agricultural modernization, has been more and more widely used in agricultural production, and has played an important role in the planting economy, especially in horticultural garden plants, Chinese herbal medicines and rare plants are even more irreplaceable, such as detoxified potato seedlings, sugarcane tissue cultures, strawberry tissue cultures, banana tissue cultures, grape tissue cultures, medicinal dendrobium tissue cultures, eucalyptus Tissue culture seedlings and many kinds of flower tissue culture seedlings and so on. [0003] The current technical problem in the field of tissue culture is how to solve the problems of low production efficiency and h...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 郝玉有
Owner 上海曼森生物科技有限公司
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