Substrate for detecting beta-D-glucuronidase and preparation method thereof as well as kit

A technology of aldolidase and glucuronic acid, which is applied to the preparation of sugar derivatives, biochemical equipment and methods, sugar derivatives, etc., can solve the problems of insensitivity to color development, difficulty in synthesis, and high price, and achieve The effect of cost reduction and simple synthesis process

Inactive Publication Date: 2018-06-08
GUANGDONG UNITY BIOTECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This substrate mainly has the following problems: 1. The color development is not sensitive, and it is easy to miss the weak positive; 2. The substrate is unstable and needs to be kept at -20°C, otherwise it is easy to deteriorate; 3. The synthesis is difficult and expensive

Method used

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  • Substrate for detecting beta-D-glucuronidase and preparation method thereof as well as kit
  • Substrate for detecting beta-D-glucuronidase and preparation method thereof as well as kit
  • Substrate for detecting beta-D-glucuronidase and preparation method thereof as well as kit

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0022] The present invention is illustrated below through specific examples, but the present invention is not limited.

[0023] 1. Synthesis of D-methyl glucuronate. It can be represented by the following reaction formula:

[0024]

[0025] The specific preparation steps are as follows: D-glucuronolactone (1) (17.6g, 0.1mol) was dissolved in 200ml methanol and cooled to 0°C. NaOH (100 mg) was added and stirred for 2 hours. The reaction was concentrated to dryness to give methyl D-glucuronate (2) as a white solid (yield 20.8 g, yield 100%).

[0026] 2. Synthesis of 1,2,3,4-tetra-O-acetyl-D-glucuronic acid methyl ester. It can be represented by the following reaction formula:

[0027]

[0028] The specific preparation steps are as follows: D-methyl glucuronate (2) (20.8g, 0.1mol) was dissolved in pyridine (45ml) and cooled to below 5°C, then acetic anhydride (54mL, 0.6mol) was added dropwise to keep it stable Not exceeding 10°C, then stirred at room temperature for 3 ...

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Abstract

The invention discloses a substrate for detecting beta-D-glucuronidase. The substrate is specifically thymolphthalein-alpha-D-glucuronic acid. A preparation method comprises the following steps: preparing thymolphthalein-alpha-D-methyl glucuronate through reaction of thymolphthalein and acetyl bromide-alpha-D-methyl gluconate, removing acetyl to obtain the thymolphthalein-alpha-D-methyl gluconate,and then performing hydrolysis reaction, so as to obtain the thymolphthalein-alpha-D-glucuronic acid. As the substrate for detecting the beta-D-glucuronidase, the thymolphthalein-alpha-D-glucuronic acid disclosed by the invention has the advantages of stable property, difficulty in deterioration and sensitivity to development; furthermore, a synthesis process is simple, and the cost can be obviously reduced.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to a substrate for detecting β-D-glucuronidase, a preparation method thereof, and a kit for detecting β-D-glucuronidase. Background technique [0002] Trichomonal vaginitis is caused by Trichomonas, a tiny flagellated protozoan organism that cannot be seen with the naked eye and needs to be observed under a microscope. The Trichomonas that parasitize the human body include Trichomonas vaginalis, Trichomonas hominis and Oral Trichomonas, which parasitize the genitourinary system, intestinal tract and oral cavity respectively. The ones related to skin diseases are Trichomonas vaginalis, which cause Trichomonas vaginalis inflammation. It is a parasitic disease that is mainly transmitted through sexual intercourse and is contagious. The vaginal pH value of patients with trichomonas vaginitis is generally 5.1 to 5.4. Trichomonas hidden in glands and vaginal folds often reproduce be...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H15/26C07H1/00C12Q1/34
CPCC07H1/00C07H15/26C12Q1/34
Inventor 苏宏文赖华梁伟业刘志文
Owner GUANGDONG UNITY BIOTECH
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