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Construction of UPLC (Ultra-Performance Liquid Chromatography) characteristic chromatogram of platycladi seed medicinal material and assay method thereof

A technology of a feature map and a construction method, which is applied in the construction and detection field of a feature map of Boziren medicinal materials, can solve the problems of inability to identify the quality of Boziren medicinal materials, and achieves the effects of good reproducibility and rich information.

Active Publication Date: 2018-06-12
GUANGDONG YIFANG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no literature report on the fingerprint / characteristic map technology research of Baiziren, and the existing standards and methods cannot effectively identify the quality of Baiziren medicinal materials

Method used

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  • Construction of UPLC (Ultra-Performance Liquid Chromatography) characteristic chromatogram of platycladi seed medicinal material and assay method thereof
  • Construction of UPLC (Ultra-Performance Liquid Chromatography) characteristic chromatogram of platycladi seed medicinal material and assay method thereof
  • Construction of UPLC (Ultra-Performance Liquid Chromatography) characteristic chromatogram of platycladi seed medicinal material and assay method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1: A method for constructing the UPLC characteristic map of the medicinal material Baiziren

[0031] 1. Instruments and reagents

[0032] Instruments: Waters H-class ultra-high performance liquid chromatography; Waters PDA detector; Empower workstation; Waters BEH (100×2.1mm, 1.7μm), Agilent SB (100×2.1mm, 1.8μm), Waters Cortecs T3 (100nm ×2.1mm×1.6μm); ten-thousandth analytical balance (ME204E, Mettler-Toledo); millionth analytical balance (XP26, Mettler-Toledo); ultrasonic cleaning machine (Wuxi Ultrasonic Electronic Equipment Factory); constant temperature water bath (Shanghai Yiheng Technology Co., Ltd., model: HWS28); ultrapure water system (Merck Co., Ltd., Milli-Q Direct).

[0033] Experimental reagents: acetonitrile (Merck, chromatographically pure); methanol (Tianjin Fuyu Chemical Reagent Co., Ltd., analytically pure); ethanol (Tianjin Fuyu Chemical Reagent Co., Ltd., analytically pure); phosphoric acid (Tianjin Kemiou Chemical Reagent Co., Ltd. Co., ...

Embodiment 2

[0126] Embodiment 2: A kind of quality detection method of the medicinal material of Baiziren

[0127] Including the following steps:

[0128] (1) Preparation of test solution

[0129] Get the sample to be tested (Boziren medicinal material, batch number: G1705136, place of origin: Liuyu Town, Pingyi County, Linyi City, Shandong Province) powder (through No. 2 sieve), accurately weighed, put in a stoppered Erlenmeyer flask, and accurately add 70 %methanol 25ml, plugged tightly, weighed, ultrasonically treated (power 250W, frequency 50KHz) for 30 minutes, allowed to cool, weighed again, supplemented the lost weight with 70% methanol, shaken, filtered, and took the filtrate , that is;

[0130] (2) Determination: Accurately draw each 1 μ l of the test solution, inject it into an ultra-high performance liquid chromatograph, measure, and obtain the characteristic spectrum of the medicinal material of Baiziren medicinal material to be tested, and the chromatographic conditions are...

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Abstract

The invention discloses the construction of a UPLC (Ultra-Performance Liquid Chromatography) characteristic chromatogram of platycladi seed medicinal material and an assay method thereof. Aiming at the problem of quality control existing in the platycladi seed medicinal material, the invention establishes the UPLC characteristic chromatogram of the platycladi seed medicinal material, and meanwhile, UPLC-MS (ultra-performance liquid chromatography-tandem mass spectrometry) is adopted to confirm the basic research of related substances, calibrating seven characteristic peaks; the UPLC characteristic chromatogram of the platycladi seed medicinal material which is established by the invention can be adopted to sufficiently display the characteristics of the chemical components of the platycladi seed medicinal material, and the amount of characteristic peak information is rich; the method has good repeatability, and is accurate and reliable, and by means of the established UPLC characteristic chromatogram, the platycladi seed medicinal material and related counterfeits can be comprehensively and effectively identified.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to the construction and detection method of a characteristic map of a medicinal material of cypress kernels. Background technique [0002] The cypress kernel is the dry mature seed kernel of the Cupressaceae plant Platycladis orientalis (L.) Franco. Sweet, flat. It has the functions of nourishing the heart and calming the nerves, moistening the intestines and laxative, and antiperspirant. Baiziren was first recorded in "Shen Nong's Materia Medica", listed as top grade, and is one of the commonly used traditional Chinese medicines in clinical practice. It has the functions of nourishing the heart and calming the nerves, antiperspirant, and moistening the intestines. It is used to treat symptoms such as insomnia, palpitations, night sweats due to yin deficiency, dryness of the intestines, and constipation. [0003] At present, the supply of Baiziren is relatively scarc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 朱德全程松刘桂联薛鹏辉陈向东
Owner GUANGDONG YIFANG PHARMA
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