Porcine reproductive and respiratory syndrome virus ELISA antibody detection kit

Abstract

A porcine reproductive and respiratory syndrome virus ELISA antibody detection kit, the kit includes: a protein mixed-encapsulated enzyme label plate, a batch pretreatment device for serum samples, a sample diluent, an enzyme-labeled conjugate, a substrate color developer, and a stop solution , Porcine Reproductive and Respiratory Syndrome positive serum and Porcine reproductive and respiratory syndrome negative serum; the protein mixed-encapsulated ELISA plate is coated with antigenic proteins: envelope protein GP5, membrane matrix protein M and nucleocapsid protein N. Compared with the existing mainstream technology that coats a single antigen, the triple-coated antigen detection antibody is more effective, which can effectively reduce the problem of detection failure or missed detection. In addition, a batch pre-processing device for serum samples was invented, which can mix serum in batches, and can use a multi-channel pipette to add samples at a time, which greatly improves efficiency and reduces errors.

Description

technical field [0001] The invention belongs to the technical field of veterinary disease detection, and relates to the preparation and use of an enzyme-linked immunosorbent assay (ELISA) kit. It can be used for the detection of reproductive and respiratory syndrome virus antibodies. Background technique [0002] Porcine Reproductive and Respiratory Syndrome (PRRS) is an acute and highly contagious viral disease caused by Porcine Reproductive and Respiratory Syndrome Virus (PRRSV), commonly known as "PRRS". It is currently one of the main diseases that mainly cause reproductive disorders and respiratory diseases in pigs. PRRS has spread all over the world's major pig-raising countries and regions. The highly pathogenic PRRS belongs to the "Class I animal disease" stipulated by the Ministry of Agriculture, which has seriously threatened the healthy development of the pig industry. Porcine reproductive and respiratory syndrome virus can be divided into North American type an...

AI Technical Summary

Problems solved by technology

Currently on the market, antibodies for detecting PRRSV are mostly coated with a single protein such as nucleocapsid protein N or polyprotein, such as the commercialized American IDEXX, which leads to missed detection of samples, low detection accuracy in the early stage of PRRSV infection, and virus mutation Defects that lead to a decrease in the detection rate

At the same time, in clinical high-throughput determination of serum antibodies, the treatment cycle of serum dilution samples is long, the workload is heavy, and the incubation time of serum samples has large errors, resulting in large errors in test results.

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