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Anti-oxidization bone collagen polypeptide and preparation method thereof

A collagen peptide and antioxidant technology, which is applied in the preparation methods of peptides, chemical instruments and methods, and food ingredients as antioxidants, etc., can solve the problems of single product source and immunogenicity.

Active Publication Date: 2018-07-06
BEIJING UNIV OF CHEM TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The present invention aims to solve the technical problem of single source of existing products and immunogenicity, and provides an antioxidant collagen polypeptide with good antioxidant performance and no immunogenicity and its preparation method

Method used

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  • Anti-oxidization bone collagen polypeptide and preparation method thereof
  • Anti-oxidization bone collagen polypeptide and preparation method thereof

Examples

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Effect test

example example 1

[0020] The preparation method provided by the invention is as follows:

[0021] (1) Extraction of tuna collagen hydrolyzate

[0022] Take 10 g of tuna bones, wash them, soak them in 1.25 mol / L hydrochloric acid for 12 hours, and change the acid every 1.5 hours to remove inorganic salts. Then the tuna bone was cleaned to a pH of 7.0, and then the enzymolysis conditions were pH 8, temperature 37°C, and enzymolysis time 2 hours, according to the conditions that the volume concentration of trypsin was 0.5%, and the volume concentration of chymotrypsin was 0.1%. trypsin and chymotrypsin, the supernatant was collected, filtered, concentrated and freeze-dried to obtain tuna collagen hydrolyzate.

[0023] (2) Separation and purification of enzymolysis products

[0024] The enzymatic hydrolysis product was separated by DEAE-52 cellulose anion collagen chromatography, and the unadsorbed components were washed away after loading the sample, and then eluted with pH 7.2 phosphate buffer ...

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Abstract

The invention relates to anti-oxidization bone collagen polypeptide and a preparation method thereof, aiming at solving the technical problems of an existing product that the source is single and theimmunogenicity exists. An amino acid sequence is shown as a sequence table 1. The invention also provides a preparation method of the anti-oxidization bone collagen polypeptide; collagen is extractedby taking tuna bones as a source; then the collagen is subjected to enzymolysis by adopting a tyrisin-chymase complex enzyme and then is separated and purified, and frozen and dried to obtain the anti-oxidization bone collagen polypeptide. The anti-oxidization bone collagen polypeptide can be used for the field of preparation of the anti-oxidization polypeptide.

Description

technical field [0001] The invention relates to a polypeptide and a preparation method thereof, in particular to an antioxidant collagen polypeptide and a preparation method thereof. Background technique [0002] Oxidation of biomolecules is a free radical-mediated process that can have many adverse effects on food and biological systems. Powerful free radicals related to various diseases such as arteriosclerosis and cancer will inevitably be produced along with the process of nourishment and metabolism. In food, the oxidation of food nutrients will produce peroxides, which will not only affect the food value, cause the quality of food to decrease, and even cause diseases in the body of the ingestor. Therefore, the search for safe antioxidants is a research hotspot in biochemical nutrition. Although synthetic antioxidants are much cheaper than natural antioxidants, they can cause harm to the human body. Therefore, it is necessary to find a safe natural antioxidant. [000...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/78C12P21/06C07K1/14C07K1/18C07K1/20C07K1/36A23L3/3535
CPCA23L3/3535A23V2002/00C07K14/78C12P21/06A23V2200/02A23V2250/55
Inventor 黄雅钦丁丁
Owner BEIJING UNIV OF CHEM TECH
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