A kind of method of hickory chick strain separation

A technology of bacterial classification separation and Morchella, applied in directions such as microorganism-based methods, separation microorganisms, and botanical equipment and methods, can solve the problems of high contamination rate, complicated operation, and many procedures of separation methods, and achieves improved separation efficiency. , Simple operation, the effect of reducing pollution

Active Publication Date: 2020-12-25
武冈市云山绿创生态农业科技发展有限公司
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Problems solved by technology

[0003] To sum up, the problems existing in the prior art are: the existing separation methods often have a high pollution rate and require repeated separation and purification for many times. After separation, the strains obtained need to undergo complex hyphae fusion, purification, cultivation identification, etc. , many procedures, complex operations, long time-consuming, etc.

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  • A kind of method of hickory chick strain separation

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Embodiment 1

[0033] The first step: production of morel mother species culture medium

[0034] A potato comprehensive medium: 200g potatoes (peeled, boiled juice), 20g glucose, 20g agar, 1g potassium dihydrogen phosphate, 0.5g magnesium sulfate, 1000mL water. Peel the potatoes, cut into pieces, add a certain amount of water, simmer the juice for 30 minutes, filter and take the juice, add agar to the juice, after simmering and melting, add glucose, potassium dihydrogen phosphate and magnesium sulfate, after dissolving, add water to make up 1000mL, Dispense while hot, and after autoclaving at 121°C for 30 minutes, pour the sterilized culture medium while hot into a 75mm-diameter petri dish on an ultra-clean workbench to make a plate culture medium for later use.

[0035] B peptone medium: peptone 20g, agar 20g, glucose 20g, wheat flour 5.0g, vitamin E 2 capsules, vitamin B 1 5 capsules, 1000mL of water. Pour an appropriate amount of water into the pot, add agar and boil it, then add pepton...

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Abstract

The invention belongs to the technical field of strain isolation, and discloses a method of morchella esculenta strain isolation. The method comprises the following steps: piercing one side of a nutrient bag for forming a hole, and placing the nutrient bag near a growing place of morchella esculenta, wherein the hole faces the ground; after 2-3 days when the hyphae of the morchella esculenta passthrough the hole of the nutrient bag and grow into a nutrient bag culture medium, taking the nutrient bag away; disinfecting the surface of the nutrient bag, taking wheat grains out through the hole,and inoculating the wheat grains into a plate culture medium; placing a culture dish containing the plate culture medium in an incubator for culture away from light, wherein the culture temperature is23 DEG C; and after 3-4 days when morchella esculenta hyphae are grown, selecting a non-polluted strain block with a size of a mung bean grain, transferring the strain into onto a new plate culture medium, and after the strain is purified, transferring the strain block into a bevel culture medium for culturing to obtain the morchella esculenta strain. When the morchella esculenta strain is isolated by using the method provided by the invention, the success rate is up to 82.69%, the pollution rate is only 17.31%, only 1-2 times of transfer are needed, the morchella esculenta strain can be obtained in 15 days, the number of transfer times can be reduced by 2-3 times on average, and the culture time can be shortened by nearly 10 days.

Description

technical field [0001] The invention belongs to the technical field of strain separation, and in particular relates to a method for separating morel strains. Background technique [0002] Morel (Morchella esculenta), belonging to Ascomycota (Ascomycota), Pezizomycetes (Pezizomycetes), Pezizales (Pezizales), Morchellaceae (Morchellaceae), commonly known as Morchella, net mushroom. It has been recognized as a precious edible and medicinal macrofungi. At present, the methods for isolation of morel strains mainly include fruiting body tissue separation method, spore separation method and morel foot soil separation method. The tissue separation method refers to the use of morel stipe or cap tissue to separate strains. However, because the fruit body of morel is hollow, the tissue structure is thin, uneven, and there are many miscellaneous bacteria, the separation process requires extremely fine. The result of separation often has a high pollution rate, and it is necessary to ca...

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Application Information

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Patent Type & AuthorityPatents(China)
IPC IPC(8): C12N1/02A01G18/40C12N1/14C12R1/645
CPCC12N1/02C12N1/14
Inventor杨顺强桑正林吴银梅程立君刘健君王世敏
Owner武冈市云山绿创生态农业科技发展有限公司