Low-iron blood-supplementing agent, black fungus melanin active ingredient F1 and use of black fungus melanin active ingredient F1

A technology of active ingredients and melanin, applied in medical preparations containing active ingredients, plant/algae/fungus/moss ingredients, blood diseases, etc., can solve problems such as intestinal flora imbalance, influence, gastrointestinal health hazards, etc. Achieve the effect of increasing hemoglobin and red blood cell count, increasing abundance, and excellent stability

Active Publication Date: 2018-07-10
HEILONGJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to solve side effects such as nausea, abdominal pain, diarrhea, constipation, intestinal flora imbalance, harm to gastrointestinal health and other side effects caused by existing iron supplements, and greatly reduce the iron content in the medicine while maintaining at least equal blood enriching effect. Iron content, to prevent iron overload from affecting the immune response of the human body, and provide low-iron blood supplements, black fungus melanin active ingredient F1 and its use

Method used

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  • Low-iron blood-supplementing agent, black fungus melanin active ingredient F1 and use of black fungus melanin active ingredient F1
  • Low-iron blood-supplementing agent, black fungus melanin active ingredient F1 and use of black fungus melanin active ingredient F1
  • Low-iron blood-supplementing agent, black fungus melanin active ingredient F1 and use of black fungus melanin active ingredient F1

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Experimental program
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specific Embodiment approach 1

[0039] Specific embodiment one: In this embodiment, black fungus melanin active ingredient F1 is prepared according to the following steps:

[0040] 1. Dissolve black fungus melanin in NaOH solution at a concentration of 5 mg / mL, and then load it on Sephadex G-200 column;

[0041] 2. Elute with PBS buffer solution with a concentration of 50mmol / L and a pH of 7.5 at a flow rate of 1.2mL / min. Collect the eluate from the 34th to 50min and adjust the pH value to 2, then let it stand, and then 4000r / min After centrifuging for 15 min, the precipitate was washed with distilled water, and then vacuum freeze-dried to obtain black fungus melanin active ingredient F1;

[0042] Wherein, the concentration of NaOH solution in step one is 0.2mol / L.

[0043] The element content of black fungus melanin and black fungus melanin active ingredient F1 is shown in Table 1. The contents of N, C, H and S in black fungus melanin are higher than that of black fungus melanin active ingredient F1. Amon...

specific Embodiment approach 2

[0051] Specific embodiment two: the difference between this embodiment and specific embodiment one is: in step two, the dextran gel Sephadex G-200 is first rinsed with a PBS buffer solution with a concentration of 50mmol / L and a pH of 7.5 and then stabilized. sample. Other steps and parameters are the same as those in Embodiment 1.

specific Embodiment approach 3

[0052] Embodiment 3: The difference between this embodiment and Embodiment 1 or 2. In Step 2, the Sephadex G-200 has a diameter of 1.6 cm and a length of 64 cm. Other steps and parameters are the same as those in Embodiment 1 or 2.

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Abstract

The invention discloses a low-iron blood-supplementing agent, a black fungus melanin active ingredient F1 and a use of the black fungus melanin active ingredient F1, and relates to the low-iron blood-supplementing agent, the black fungus melanin active ingredient F1 and the use of the black fungus melanin active ingredient F1. The problems that a conventional iron supplementing agent can cause diarrhea, constipation, maladjustment of intestinal flora, harm to gastrointestinal health, anaphylactic shock and other side effects, or is expensive, is limited in effect of anemia improvement and hasthe disease easy to relapse are solved. The preparation method of the black fungus melanin active ingredient F1 comprises the steps: one, dissolving black fungus melanin in a NaOH solution, and then loading the sample in a dextran gel column; and two, eluting with a PBS buffer solution, collecting an eluate at 34th-50th min, adjusting the pH value, allowing to stand, centrifuging, and carrying outvacuum freeze drying. The black fungus melanin active ingredient F1 can obviously improve the number of hemoglobin and haemocytes in an IDA mouse model, improves haemopoietic factors, increases ironabsorption, improves iron-deficiency anemia effectively, and also cannot cause nausea, abdominal pain, diarrhea, constipation and other adverse symptoms.

Description

technical field [0001] The invention relates to a low-iron blood-enriching agent, black fungus melanin active ingredients and uses thereof. Background technique [0002] Iron deficiency anemia (Iron Deficiency Anemia, IDA) is an anemia caused by the depletion of iron reserves in the human body resulting in decreased hemoglobin synthesis. This type of anemia is characterized by a lack of stainable iron in tissues such as bone marrow, liver, and spleen, and decreased serum iron, transferrin saturation, and ferritin. There are many causes of iron deficiency anemia, which are generally summarized as follows: (1) increased iron requirement, which mostly occurs in infants and pregnant women; (2) insufficient iron intake and malabsorption, resulting in insufficient iron intake. The reason is insufficient iron content in food, partial eclipse, phosphate, phytic acid, and tannic acid in vegetables, grains, and tea in food will reduce the absorption of iron in the gastrointestinal tr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/07A61P7/06A61P1/00
CPCA61K36/07A61K2236/55A61K2236/51A61K2236/53A61K2236/333
Inventor 雷虹曾伟民万鹏江伟冯磊程文诸琳王淼
Owner HEILONGJIANG UNIV
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