The invention discloses a preparation method of spinach handmade macaroni. The preparation method comprises the following steps of adding table salt and water to flour and spinach juice, then performing kneading to obtain dough, and flattening the obtained dough for standing; making flour and corn flour into dry powder, cutting the fermented dough into strips, then repeatedly twisting the strips into thick strips, coiling the thick strips, laying and sprinkling dry powder on each layer of the coiled thick strips, and allowing the coiled thick strips sprinkled with the dry powder to stand; then twisting the thick strips into fine strips, coiling the fine strips, laying and sprinkling the dry powder on each layer of the coiled fine strips, and allowing the coiled fine strips sprinkled with the dry powder to stand; enabling noodles to be wound on two noodle hanging rods, horizontally placing one of the two noodle hanging rods in a noodle leavening bin, and vertically hanging the other noodle hanging rod in the noodle leavening bin for standing; fixing one of the two noodle hanging rods on a noodle separating rack, repeatedly stretching noodles through the other noodle hanging rod, separating noodles with a noodle separating rod, drawing out the noodle hanging rod at the lower part, and putting the drawn-out noodle hanging rod and the noodle hanging rod at the upper part back into the noodle leavening bin side by side for standing; and naturally airing hung noodles, cutting the aired noodles, and performing packaging. According to the preparation method disclosed by the invention, based on conventional handmade fine dried noodles, research, development and creation are performed, so that nutritive intake in spinach required for human bodies reaches high standards.