sgRNA and its prepared Gal antigen-deficient rabbit model and its application

Abstract

The invention relates to a sgRNA and a Gal antigen-deficient rabbit model prepared by the same and its application, belonging to the technical field of animal model establishment. The sgRNA is aimed at the GGTA gene of rabbit species, and its genome number is LOC100348435. 20 The sequence parts of the AGG sequence pattern are respectively designed recognition sequences, and the sgRNAs are respectively recorded as the first sgRNA recognition sequence and the second sgRNA recognition sequence. The Gal antigen-deficient rabbit model prepared by using the Cas9-GGTA / sgRNA, its homozygous lost the ability to express the Gal antigen, and has a similar expression trend of anti-Gal antibodies to the human body; therefore, the Gal-antigen-deficient rabbit model can be used Immunological research on animal-derived biological materials, animal tissues, organs and their derivatives, host implantation response evaluation of animal-derived medical products, calcification test research on animal-derived membrane biomaterials and implanted animal-derived Evaluation of the effectiveness of medical products based on biomaterials.

Description

technical field [0001] The invention relates to the technical field of animal model establishment, in particular to a sgRNA and a Gal antigen-deficient rabbit model prepared by the sgRNA and its application. Background technique [0002] Studies have shown that the xenoantigen α-galactosyl antigen (α-1,3-galactosyle, α-Gal, ie: Gal antigen) is the main target of hyperacute immune rejection of animal-derived biological materials or xenogeneic organ transplantation. antigen. Gal antigen is a cell surface secreted glycoprotein or glycolipid containing polylactosamine core terminal residues, which widely exists in lower animals such as pigs and rabbits. Gal antigen is mainly regulated by α-1,3-galactosyltransferase (α-1,3-galactosyltransferase, α-1,3GT or GGTA1). Because the galactosyltransferase gene of the human body, great apes, and old century monkeys has a 2-base dislocation mutation and does not express the Gal antigen, there are high titers of anti-Gal antibodies in hum...

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Problems solved by technology

[0004] However, there is no report on the successful preparation of GGTA1 gene knockout rabbits, and there is an urgent need for a kind of immunological research that can be used for xenogeneic tissue / organ transplantation such as pigs and cattle, animal-derived biological materials, animal tissues, organs and their derivatives. GGTA1 Gene Knockout Rabbit Model with Gal Antigen Deficiency

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