Method of tissue culture for pepper

A tissue culture, pepper technology, applied in horticultural methods, botanical equipment and methods, gardening, etc., can solve problems such as hindering pepper gene transformation

Inactive Publication Date: 2018-07-31
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although there are a series of publications on exploring and researching the construction of pepper regeneration system, in fact, pepper crops have not established a mature and stable regeneration system, which has become a major obstacle to the transformation of pepper genes.

Method used

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  • Method of tissue culture for pepper
  • Method of tissue culture for pepper
  • Method of tissue culture for pepper

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Experimental program
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Effect test

Embodiment 1

[0049] Embodiment 1, the establishment of the tissue culture method of capsicum

[0050] Experimental materials: Q7 and Q8.

[0051] 1. Soak the seeds of the experimental material in an aqueous solution containing 1g / L chlorothalonil for 5 minutes, then soak them in 75% (volume percent) ethanol aqueous solution for 30 seconds, and then transfer to 8-10% Shake and disinfect in sodium hypochlorite solution for 10-20 minutes, and finally wash with sterile water 4 times, each time for 3-5 minutes.

[0052] 2. After completing step 1, inoculate the seeds in 1 / 2MS solid medium, and cultivate in the dark at 25-28°C until the seeds germinate. After germination, adjust the light conditions to 16h light (1600-1800lx) / 18h dark, and continue Cultivate to seedling age 8-10 days.

[0053] 3. After completing step 2, cut cotyledons with a side length of 0.5-0.7 cm and inoculate them in induction medium I (containing 1.8 mg / L TDZ, 0.2 mg / L NAA and 2.0 mg / L AgNo 3 MS solid medium) or induct...

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Abstract

The invention discloses a method of tissue culture for a pepper. The method of the tissue culture for the pepper comprises the following steps that (1) pepper cotyledon cutting pieces are taken as explants, adventitious bud induction culture is carried out to obtain adventitious buds; (2) after step (1) is completed, the adventitious buds with length greater than 2 centimeters are selected for rooting culture, and regeneration plants are obtained. An inventor of the method of the tissue culture for the pepper performs repeated induction regeneration tests on different tissues of pepper cotyledon, hypocotyledonary axis, cotyledon with diseases, true leaves and the like, finally, the method of the tissue culture taking the pepper cotyledon as the explants and capable of making the pepper adventitious buds efficiently elongating is screened out, and an efficient regeneration system of the pepper is established successfully. The method of the tissue culture is of great significance for thegenetic transformation and variety improvement of the pepper, and has a wide application prospect.

Description

technical field [0001] The invention relates to a method for tissue culture of pepper. Background technique [0002] As the key plant biotechnology, tissue culture and DNA recombination technology are powerful tools to make up for the shortage of conventional crop breeding and to promote the process of crop breeding. However, capsicum is a very stubborn Solanaceae crop, and its "stubbornness" is reflected in many aspects such as cell culture, tissue culture, organ differentiation, plant regeneration and genetic transformation, making it possible to carry out effective genetic improvement of capsicum. Advances in recombinant technology have been very difficult (slow). The construction of plant tissue culture regeneration system is the premise of gene transformation. Although there are a series of literatures published on exploring the construction of pepper regeneration system, in fact pepper crops have not established a mature and stable regeneration system, which has beco...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 张晓芬耿三省陈斌杜和山
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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