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A kind of concentrated nano fat and its preparation method and application

A fat and nanotechnology, applied in medical science, prosthesis, etc., can solve the problems of increasing the risk of contamination, prolonging the operation time, and limiting clinical development, so as to achieve no immune rejection, improve extraction efficiency, and reduce component pollution. Effect

Active Publication Date: 2020-12-11
JINAN UNIVERSITY
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  • Abstract
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  • Claims
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AI Technical Summary

Problems solved by technology

In the action system of fat, SVF and extracellular matrix play their respective roles. The clinical development of SVF / adipocyte stem cell therapy is limited, largely due to the application of collagenase, and there are many reasons for using fat instead of cell therapy alone. Problems with Complications from High Fat Content
In 2013, Tonnard et al proposed the new concept of large particle fat, small particle fat and nano fat, among which nano fat has been widely used due to its good regenerative function, but the survival rate and complications of fat are still problems to be solved
However, during the operation, they separated the upper layer of oil in advance and mixed it at the end. These cumbersome steps not only prolong the operation time, but also increase the risk of contamination

Method used

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  • A kind of concentrated nano fat and its preparation method and application
  • A kind of concentrated nano fat and its preparation method and application
  • A kind of concentrated nano fat and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Abdominal Fat Extraction from New Zealand Rabbits

[0045] (1) Centrifuge to remove water: After routine disinfection of New Zealand rabbits, use a 10ml syringe to obtain 50ml of small particles of fat on the back of the rabbit, centrifuge the small particles of fat with a centrifugal force of 1000g for 3min, remove the lower liquid layer, and place the fat on ice cubes Stand still in an ice bucket, keep the temperature of small particles of fat at 4°C, and after standing for about ten minutes, the fat and swelling fluid layers can be clearly separated, and the liquid layer should be removed. Remove the lower liquid layer.

[0046] (2) Production of nano-fat: first cut the fat obtained in step (1) 30 times with a 2.4mm double-nut connector, then cut 30 times with a 1.2mm double-nut connector, and then filter with a 500-mesh screen .

[0047] (3) Negative pressure centrifugation: Connect two 10ml spiral syringes through a syringe converter, place 5ml of the fat obtaine...

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Abstract

The invention discloses a concentrated nanofat, and a method of preparing the concentrated nanofat. The method includes the following steps: (1) centrifugation for removing water, (2) preparation of nanofat, (3) negative pressure centrifugation, and (4) secondary centrifugation. The invention also discloses an application of the concentrated nanofat in the preparation of a stromal vascular fraction. The invention further discloses an application of the concentrated nanofat in the preparation of platelet-rich blood plasma. The invention further discloses a concentrated nanofat compounded platelet-rich plasma transplanting material.

Description

technical field [0001] The invention belongs to the field of tissue engineering and biomaterials, in particular to a concentrated nano fat. Background technique [0002] Because fat contains active substances such as stem cells and extracellular matrix, it has been widely used in the field of clinical regeneration and repair. In the action system of fat, SVF and extracellular matrix play their respective roles. The clinical development of SVF / adipocyte stem cell therapy is limited, largely due to the application of collagenase, and there are many reasons for using fat instead of cell therapy alone. The problem of complications caused by high oil content. In 2013, Tonnard et al. put forward the new concept of large particle fat, small particle fat and nano-fat. Among them, nano-fat has been widely used due to its good regenerative function, but the survival rate and complications of fat are still problems to be solved. In 2017, Lu Feng et al. used a special device to remove...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/36
CPCA61L27/3604A61L27/3616A61L27/3691
Inventor 刘宏伟吴引弟廖选蒋笑李泽华
Owner JINAN UNIVERSITY
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