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A kind of triterpenoid compound in Chengguang flower and its preparation method and application

A technology of triterpenoids and compounds, applied in the field of triterpenoids and their preparation, can solve problems such as no relevant reports on the application, and achieve good application prospects

Active Publication Date: 2020-11-10
CHINA TOBACCO YUNNAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no relevant report on the compound and its application

Method used

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  • A kind of triterpenoid compound in Chengguang flower and its preparation method and application
  • A kind of triterpenoid compound in Chengguang flower and its preparation method and application
  • A kind of triterpenoid compound in Chengguang flower and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] A preparation method of triterpenoids in Cheng Guang Hua, comprising the steps of:

[0044] Step (1), extraction of extract: crush the dried Chengguanghua into 30-40 meshes, extract 2 times at room temperature with 70% ethanol aqueous solution at room temperature, combine the extracts, and filter to obtain Filtrate is concentrated under reduced pressure to obtain medicinal extract; The volume of ethanol aqueous solution and the mass ratio of Cheng Guanghua are 8L: 1Kg when extracting each time;

[0045] Step (2), silica gel column chromatography: the extract obtained in step (1) is first dissolved with a volume concentration of 1.5 times the weight of the extract as a 95% aqueous methanol solution, and then stirred with 100 mesh silica gel with 2 times the weight of the extract Mix well, then load the sample for silica gel column chromatography, the silica gel used for packing the column is 200-250 mesh, and the weight of silica gel used for packing the column is 3 time...

Embodiment 2

[0050] A preparation method of triterpenoids in Cheng Guang Hua, comprising the steps of:

[0051] Step (1), extraction of extract: crush the dried Chengguanghua into 40-50 meshes, extract 4 times at room temperature with 95% ethanol aqueous solution at room temperature, combine the extracts, and filter to obtain Filtrate is concentrated under reduced pressure to obtain medicinal extract; The volume of ethanol aqueous solution and the mass ratio of Cheng Guanghua are 1-2L:1Kg when extracting each time;

[0052] Step (2), silica gel column chromatography: the extract obtained in step (1) is first dissolved with a volume concentration of 4 times the weight of the extract as a 95% aqueous methanol solution, and then stirred with 100 mesh silica gel with 4 times the weight of the extract Mix well, then load the sample for silica gel column chromatography, the silica gel used for packing the column is 250-300 mesh, the weight of silica gel used for packing the column is 5 times the...

Embodiment 3

[0058] A preparation method of triterpenoids in Cheng Guang Hua, comprising the steps of:

[0059] Step (1), extraction of extract: crush the dried Chengguanghua into 30-50 meshes, extract 3 times with 90% ethanol aqueous solution at room temperature, each time for 8 hours, combine the extracts, and filter to obtain Filtrate is concentrated under reduced pressure to obtain extract; the volume of ethanol aqueous solution and the mass ratio of Cheng Guanghua are 10 L:1Kg during each extraction;

[0060] Step (2), silica gel column chromatography: first dissolve the extract obtained in step (1) with a volume concentration of 2 times the weight of the extract as a 95% aqueous methanol solution, and then stir with 100 mesh silica gel with 3 times the weight of the extract Mix well, then load the sample for silica gel column chromatography, the silica gel used for packing the column is 220-280 mesh, and the weight of silica gel used for packing the column is 4 times the weight of th...

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Abstract

The invention relates to a triterpenoid compound in orophea yunnanensis as well as a preparation method and application of the triterpenoid compound, and belongs to the technical field of phytochemistry. The compound is named as 3-(4'-methoxy-5',6'-dihydroxybenzaldehyde)-3-beta-oleanol-14-ene, has a molecular formula of C37H54O4 and is shown in a formula (I) in the description. The compound provided by the invention is separated from branches and leaves of the orophea yunnanensis in Xishuangbanna, Yunnan for the first time, the compound is identified as the triterpenoid compound through nuclear magnetic resonance and mass spectrometric measurement methods, and a specific structure of the structure is characterized; the compound shows moderate inhibitory activity against rape sclerotinia sclerotiorum, alternaria fragariae and potato verticillium wilt pathogens, and a minimum inhibitory concentration is 25-100 [mu]g / mL; and meanwhile, the compound shows weak antibacterial activity to some bacteria, a minimum inhibitory concentration is 50-100 [mu]g / mL, and the compound has good application prospects.

Description

technical field [0001] The invention belongs to the technical field of phytochemistry, and in particular relates to a triterpenoid compound in Chengguang flower and its preparation method and application. Background technique [0002] Orophea yunnanensis is a plant of the genus Orophea yunnanensis in the family Anemonaceae, which is produced in central Yunnan. Chengguang flower is rich in triterpenoid active ingredients. Triterpenoids are a kind of terpenoids whose basic nucleus is composed of 30 carbon atoms. They exist in free form or in the form of glycosides or esters combined with sugar. Plants have various biochemical activities. The present invention systematically separates the triterpenoid active components of the plant to obtain a triterpenoid active component with a novel structure. At the same time, using the activity test platform, it is found that it is effective against bacteria and fungi. All have certain activity performance, which provides basic data for t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07J63/00A01N37/40A01P1/00A01P3/00
CPCA01N37/40C07J63/008
Inventor 杨柳赵伟洪鎏杨继段沅杏巩效伟田永峰赵杨朱东来陈永宽
Owner CHINA TOBACCO YUNNAN IND
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