Application of long-chain non-coding RNA LINC00336 serving as biomarker to preparation of lung adenocarcinoma prognostic detection preparation

A long-chain non-coding and biomarker technology, applied in the field of preparation of lung adenocarcinoma prognosis detection reagents, can solve the problem of not being able to adapt to the prognosis of lung adenocarcinoma patients, there is no reference standard for the prognosis of lung adenocarcinoma patients, and there is no specificity issues such as specific indicators, to achieve far-reaching clinical significance and promotional effects

Active Publication Date: 2018-08-28
XIANGYA HOSPITAL CENT SOUTH UNIV +1
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  • Abstract
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  • Application Information

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Problems solved by technology

[0004] At present, there is no reference standard for the prognosis of patients with lung adenocarcinoma, and ...

Method used

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  • Application of long-chain non-coding RNA LINC00336 serving as biomarker to preparation of lung adenocarcinoma prognostic detection preparation
  • Application of long-chain non-coding RNA LINC00336 serving as biomarker to preparation of lung adenocarcinoma prognostic detection preparation
  • Application of long-chain non-coding RNA LINC00336 serving as biomarker to preparation of lung adenocarcinoma prognostic detection preparation

Examples

Experimental program
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Embodiment 1

[0013] Example 1 Preparation of long-chain non-coding RNA LINC00336 for lung adenocarcinoma prognosis detection kit (50 reactions)

[0014] 1. RNA stabilization solution 50ml

[0015] 2. Isopropanol 100ml

[0016] 3. Chloroform 100ml

[0017] 4. Trizol 50ml

[0018] 5. Enzyme-free water 10ml

[0019] 6.1μM random reverse transcription primer 50ul

[0020] 7. 5× reverse transcription buffer 200ml

[0021] 8. 10mM base triphosphate deoxyribonucleotides 100ul

[0022] 9. 40U / μl RNase inhibitor 500ul

[0023] 10. 200U / μl MMLV reverse transcriptase 50ul

[0024] 11. Premix Ex Taq 50ul

[0025] 12. 10μM long non-coding RNA LINC00336 real-time fluorescent quantitative PCR specific primers:

[0026] Forward primer 5'-AGCAGAGGTGCTGGCCAAGGCGCTC-3' (SEQ ID NO.2),

[0027] Reverse primer 5'-CTATAATCCTAGCACTTTCGGAGGC-3' (SEQ ID NO.3),

[0028] 13. 10μM internal reference GAPDH specific primers for real-time fluorescence quantitative PCR:

[0029] The forward primer is 5'-GTCTCC...

Embodiment 2

[0031] Example 2 Detection of long non-coding RNA LINC00336 in lung adenocarcinoma tissue

[0032] 1. Preservation of lung adenocarcinoma tissue: collect the lung adenocarcinoma tissue to be tested and store it in a cryopreservation tube filled with RNA stabilization solution, and put it in a -80°C refrigerator for later use.

[0033] 2. Extraction of RNA in tissues: Take an appropriate amount of specimen, add liquid nitrogen to the mortar after baking at 180°C for 6-8 hours, grind the specimen, grind to powder, add 1ml Trizol mortar specimen to the mortar, and grind into After the liquid is in the centrifuge tube, add 200μl / ml chloroform to the centrifuge tube, shake it by hand for 15-30s, place it on ice for 5min, and centrifuge at 12000g at 4°C for 15min; carefully take the upper aqueous phase into a new centrifuge tube, add Mix with 0.5ml of pre-cooled isopropanol, put it in a refrigerator at -20°C for 20min, centrifuge at 12000g at 4°C for 10min; discard the supernatant, ...

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Abstract

The invention discloses the application of long-chain non-coding RNA LINC00336 serving as a biomarker to the preparation of a lung adenocarcinoma prognostic detection preparation. The expression levelof the long-chain non-coding RNA LINC00336 in a patient suffering from lung adenocarcinoma can be detected, a powerful molecular biology base is provided for prognostic prediction of the patient suffering from lung adenocarcinoma, and profound clinical significance and generalization performance are achieved.

Description

technical field [0001] The invention belongs to the field of tumor molecular biology, and specifically relates to the application of long-chain non-coding RNA LINC00336 as a biomarker in the preparation of lung adenocarcinoma prognosis detection reagents. Background technique [0002] Lung cancer is the leading cause of cancer-related death worldwide, accounting for 28% of cancer deaths in men and 26% in women. Despite the rapid progress in clinical medicine and experimental oncology related to lung cancer, the prognosis of lung cancer is not satisfactory, and the 5-year overall survival rate is nearly 11%. At the beginning of the 21st century, the World Health Organization announced the prevalence of different types of lung cancer. Among them, lung adenocarcinoma ranked first, with an incidence rate of 31.5%, followed by lung squamous cell carcinoma, with an incidence rate of 29.4%, and small cell lung cancer. The rate was 17.8%, and the incidence rate of large cell lung c...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886
CPCC12Q1/6886C12Q2600/118C12Q2600/158C12Q2600/166C12Q2600/178
Inventor 陶永光王敏毛超刘双
Owner XIANGYA HOSPITAL CENT SOUTH UNIV
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