Method for detecting concentration of tigecycline in blood plasma

A technology of tigecycline and plasma, which is applied to measurement devices, instruments, scientific instruments, etc., can solve the problem that municipal hospitals do not have mass spectrometer detectors, etc., and achieve the effects of high sensitivity and low cost

Inactive Publication Date: 2018-08-31
LIUZHOU CITY HEALTHCARE HOSPITAL FOR WOMEN & CHILDREN
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Problems solved by technology

[0003] At present, there have been reports in China on the determination of tigecycline concentration in human serum by high performance liquid chromatography tandem mass spectrometry, but most municipal hospitals do not have mass spectrometry detectors, which limits the application of high performance liquid chromatography tandem mass spectrometry

Method used

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  • Method for detecting concentration of tigecycline in blood plasma
  • Method for detecting concentration of tigecycline in blood plasma
  • Method for detecting concentration of tigecycline in blood plasma

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Embodiment 1

[0034] 1. Materials and methods

[0035] 1.1 Instrument

[0036] High performance liquid chromatography (Shimadzu LC-20AT pump, SPD-20A detector, CTO-10ASvp chromatographic column box, SIL-20A autosampler, CBM-20A system controller); electronic balance; PHS-3SPH meter ( Shanghai Jingke); XW-80A vortex mixer (Shanghai Qingpu West Instrument Factory); high-speed centrifuge (Hangzhou Aosheng Instrument Co., Ltd.).

[0037] 1.2 Reagent

[0038] Tigecycline standard substance (purity: 99.7%, China Institute for Food and Drug Control, target substance, batch number: 20305RS1505123), ranitidine standard substance (purity: 99.1%, internal standard, China Food and Drug Control Institute, batch number : 100163-201006), methanol (chromatographically pure, TEDIA), acetonitrile (chromatographically pure, TEDIA), formic acid (analytical pure, Sinopharm Chemical Reagent Co., Ltd.), perchloric acid, calf serum (Zhejiang Tianhang Biotechnology Co., Ltd. , grade: super, batch number: 130919)...

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Abstract

The invention belongs to the technical field of in-vivo medicine detection, in particular to a method for detecting the concentration of tigecycline in blood plasma. According to the method for detecting the concentration of tigecycline in the blood plasma, ranitidine is adopted as an internal standard substance, and a high-performance liquid chromatograph is adopted for calculating the concentration of tigecycline in the blood plasma according to peak areas by means of an internal standard method under a certain chromatographic condition. The high-performance liquid chromatograph is adopted for detecting the concentration of tigecycline in the blood plasma, and the detection limitation can reach 0.028 microgram/mL. The method has the advantages of being high in accuracy and sensitivity, simple, high in detection speed and the like.

Description

【Technical field】 [0001] The invention relates to the technical field of in vivo drug detection, in particular to a method for detecting the concentration of tigecycline in blood plasma. 【Background technique】 [0002] Tigecycline (TGC) is a glycylphthalein tetracycline antibacterial drug, which binds to the 305 subunit of the bacterial ribosome to block protein synthesis, thereby exerting an antibacterial effect. TGC is mainly used clinically to treat a variety of serious infections caused by Gram-positive bacteria and Gram-negative bacteria (except Pseudomonas aeruginosa), especially serious infections caused by multidrug-resistant bacteria and pan-drug-resistant bacteria . TGC is widely distributed after entering the human body, 59% of its dose is excreted through bile, and 33% is excreted through the kidneys. Patients with hepatic insufficiency need to adjust the dose, while patients with renal insufficiency do not need to adjust the dose. The situation of bacterial dr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 冯泽英覃倩婷
Owner LIUZHOU CITY HEALTHCARE HOSPITAL FOR WOMEN & CHILDREN
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