Propagation method of jasmine
A jasmine and subculture multiplication technology, which is applied in the field of plant propagation, can solve the problems of single jasmine cultivar, low natural seed setting rate of jasmine, and reduced flower yield, and achieve the effects of shortening the seedling slowing time, high survival rate, and vigorous growth
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Embodiment 1
[0018] A kind of propagation method of jasmine, comprises the following steps:
[0019] Pretreatment: Select freshly extracted jasmine shoots with a length of 8 cm, rinse with neutral detergent for 1 minute, then rinse with running water for 25 minutes, then sterilize with 85% alcohol for 8 seconds, rinse with sterile water for 3 times, and then use Sterilize with 0.08% mercuric chloride solution for 8 minutes, rinse with sterile water 3 times, and aseptically peel off the bud tip;
[0020] Induction culture: prepare the induction medium as follows: agar 1.2g / L, sucrose 5g / L, coconut milk 60g / L, folic acid 2.0mg / L, naphthalene acetic acid 3.5mg / L, insert a glass rod into a 2-3cm deep hole , vertically insert a small piece of bud tip into each small hole, evenly spread 1mm thick powdered activated carbon, and cultivate it for 25 days under the culture conditions of temperature 23°C, light time 8 hours, and light intensity 1500Lux. to sprout;
[0021] Subculture proliferation ...
Embodiment 2
[0025] A kind of propagation method of jasmine, comprises the following steps:
[0026]1) Select newly extracted jasmine sprouts with a length of 12cm, rinse with neutral detergent for 2 minutes, then rinse with running water for 30 minutes, then sterilize with 95% alcohol for 10 seconds, rinse with sterile water 5 times, and then rinse with 0.1 % mercuric chloride solution for 10 minutes, rinsed with sterile water for 5 times, and aseptically stripped the bud tips; 2) Prepare induction medium: agar 1.5g / L, sucrose 8g / L, coconut juice 80g / L, folic acid 3.0mg / L, naphthaleneacetic acid 4.0mg / L, insert a glass rod into a 3cm deep hole, vertically insert 2 pieces of bud tip small pieces into each hole, evenly spread 1-3mm thick powdered activated carbon, The temperature is 25°C, the light time is 10 hours, and the light intensity is 2000Lux, and the culture conditions are 35 days to obtain clustered buds; 3) The preparation of subculture proliferation medium is: agar 5g / L, sucrose...
Embodiment 3
[0028] A propagation method of jasmine, comprising: pretreatment, induction culture, subculture multiplication culture, rooting culture, seedling hardening and transplanting, specifically comprising the following steps:
[0029] Pretreatment: select freshly extracted jasmine sprouts with a length of 10cm, rinse with neutral detergent for 2 minutes, then rinse with running water for 25 minutes, then sterilize with 90% alcohol for 10 seconds, rinse with sterile water for 3 times, and then use Sterilize with 0.1% mercuric chloride solution for 10 minutes, rinse with sterile water 3 times, and aseptically strip off the bud tip; explant sterilization can kill harmful microorganisms carried by the bud tip of jasmine and avoid introducing harmful microorganisms into the culture medium Cause pollution, and at the same time, it can avoid the absorption and utilization of microorganisms on the culture medium, and provide sufficient nutrients for the bud tip cells for their differentiatio...
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