Propagation method of jasmine

A jasmine and subculture multiplication technology, which is applied in the field of plant propagation, can solve the problems of single jasmine cultivar, low natural seed setting rate of jasmine, and reduced flower yield, and achieve the effects of shortening the seedling slowing time, high survival rate, and vigorous growth

Inactive Publication Date: 2018-09-14
金华市野泉园艺技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the jasmine cultivar is single and the natural seed-setting rate of jasmine is low, such as the double-lobe jasmine is only 0.19%; the offspring of sexual reproduction are seriously separated, and cutting propagation is often used in production, but long-term asexual reproduction leads to the degradation of jasmine species and the stress resistance. Decline, flower production decreases year by year

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A kind of propagation method of jasmine, comprises the following steps:

[0019] Pretreatment: Select freshly extracted jasmine shoots with a length of 8 cm, rinse with neutral detergent for 1 minute, then rinse with running water for 25 minutes, then sterilize with 85% alcohol for 8 seconds, rinse with sterile water for 3 times, and then use Sterilize with 0.08% mercuric chloride solution for 8 minutes, rinse with sterile water 3 times, and aseptically peel off the bud tip;

[0020] Induction culture: prepare the induction medium as follows: agar 1.2g / L, sucrose 5g / L, coconut milk 60g / L, folic acid 2.0mg / L, naphthalene acetic acid 3.5mg / L, insert a glass rod into a 2-3cm deep hole , vertically insert a small piece of bud tip into each small hole, evenly spread 1mm thick powdered activated carbon, and cultivate it for 25 days under the culture conditions of temperature 23°C, light time 8 hours, and light intensity 1500Lux. to sprout;

[0021] Subculture proliferation ...

Embodiment 2

[0025] A kind of propagation method of jasmine, comprises the following steps:

[0026]1) Select newly extracted jasmine sprouts with a length of 12cm, rinse with neutral detergent for 2 minutes, then rinse with running water for 30 minutes, then sterilize with 95% alcohol for 10 seconds, rinse with sterile water 5 times, and then rinse with 0.1 % mercuric chloride solution for 10 minutes, rinsed with sterile water for 5 times, and aseptically stripped the bud tips; 2) Prepare induction medium: agar 1.5g / L, sucrose 8g / L, coconut juice 80g / L, folic acid 3.0mg / L, naphthaleneacetic acid 4.0mg / L, insert a glass rod into a 3cm deep hole, vertically insert 2 pieces of bud tip small pieces into each hole, evenly spread 1-3mm thick powdered activated carbon, The temperature is 25°C, the light time is 10 hours, and the light intensity is 2000Lux, and the culture conditions are 35 days to obtain clustered buds; 3) The preparation of subculture proliferation medium is: agar 5g / L, sucrose...

Embodiment 3

[0028] A propagation method of jasmine, comprising: pretreatment, induction culture, subculture multiplication culture, rooting culture, seedling hardening and transplanting, specifically comprising the following steps:

[0029] Pretreatment: select freshly extracted jasmine sprouts with a length of 10cm, rinse with neutral detergent for 2 minutes, then rinse with running water for 25 minutes, then sterilize with 90% alcohol for 10 seconds, rinse with sterile water for 3 times, and then use Sterilize with 0.1% mercuric chloride solution for 10 minutes, rinse with sterile water 3 times, and aseptically strip off the bud tip; explant sterilization can kill harmful microorganisms carried by the bud tip of jasmine and avoid introducing harmful microorganisms into the culture medium Cause pollution, and at the same time, it can avoid the absorption and utilization of microorganisms on the culture medium, and provide sufficient nutrients for the bud tip cells for their differentiatio...

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PUM

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Abstract

The invention discloses a propagation method of jasmine. The propagation method comprises the following steps: pretreatment, induced culture, subculture multiplication culture, rooting culture, and seedling exercise transplanting. Firstly, new jasmine buds are selected, rinsed and sterilized to collect the shoot tips, multiple shoots can be obtained from the shoot tips by virtue of the induced culture, the multiple shoots are cut to collect bud segments, then the subculture multiplication culture is performed for the shoot segments, seedlings are cut to have the rooting culture, and after theseedling exercise, the seedlings are transplanted. The propagation method has the beneficial effects that the propagation method is simple to implement, various varieties of the jasmine can be propagated, not only can the stable inheritance of genetic substances be realized, but also the multiple shoots can be differentially cultured for multiple times and scores of times, so that the propagationefficiency is greatly improved, the cost is reduced, the jasmine seedling root is developed, the survival rate is high, the seedling can be survived rapidly and can grow rapidly, and the plant is strong and vigorous in growth.

Description

technical field [0001] The invention relates to the field of plant propagation, in particular to a jasmine propagation method. technical background [0002] Jasmine, alias: Jasmine, Latin name: Jasminum sambac (L.) Ait , Oleaceae, Frangipani are erect or climbing shrubs, up to 3 meters. Branchlets terete or slightly compressed, sometimes hollow, sparsely pilose. Leaves are opposite, simple, papery, round, oval, ovate-elliptic or obovate, with rounded or blunt ends, sometimes slightly cordate base, slightly concave or concave above, convex below, thin Veins are often conspicuous on both sides, slightly raised, glabrous except for tufts in the axils of lower veins; lobes oblong to nearly round, apex round or obtuse. Fruit spherical, purple-black. The flowering period is from May to August, and the fruiting period is from July to September. The flowers of jasmine are very fragrant, and it is a famous scented tea raw material and an important essence raw material; the flowe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 王志刚
Owner 金华市野泉园艺技术有限公司
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