Constructing method for subacute hyperuricemia renal damage mouse model

A hyperuricemia and mouse model technology, applied in the direction of pharmaceutical formulas, preparations for in vivo tests, medical preparations containing active ingredients, etc., can solve problems such as methods that do not conform to clinical pathogenesis, and achieve serum uric acid levels Stable, stable effect

Inactive Publication Date: 2018-09-14
WUHAN POLYTECHNIC UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The main purpose of the present invention is to propose a method for constructing a mouse model of subacute hyperuricemia renal damage, aiming to solve the problem that the existing methods of hyperuricemia renal damage mouse model do not conform to the clinical pathogenesis

Method used

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  • Constructing method for subacute hyperuricemia renal damage mouse model
  • Constructing method for subacute hyperuricemia renal damage mouse model
  • Constructing method for subacute hyperuricemia renal damage mouse model

Examples

Experimental program
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Embodiment 1

[0047] Example 1 Construction of subacute hyperuricemia renal damage mouse model

[0048] 1. Selection of model animals: 30 male Kunming mice of SPF grade, weighing 25-30 g, provided by the Experimental Animal Center of Huazhong University of Science and Technology, the animal production license number is SCXK (E) 2016-0009, and the breeding environment temperature is 22 ~26°C, humidity 40-70%, mice free access to food and water.

[0049] 2. Drug solution preparation: the yeast extract (batch number 20160628, purchased from Beijing Aoboxing Biotechnology Co., Ltd.) was prepared into a yeast extract suspension with a yeast extract concentration of 1.5 g / mL using 0.5% CMC-Na aqueous solution; Potassium oxonate (batch number P137112, purchased from Shanghai Aladdin Biochemical Technology Co., Ltd.) was prepared with 0.5% CMC-Na aqueous solution to prepare a potassium oxonate suspension with a potassium oxonate concentration of 20 mg / mL; and , the yeast extract and adenine (batch...

Embodiment 2

[0066] Example 2 Verification of Allopurinol on the Mouse Model of Subacute Hyperuricemia Renal Damage

[0067] 1. Selection of model animals: 30 male Kunming mice of SPF grade, weighing 18-22 g, provided by the Experimental Animal Center of Huazhong University of Science and Technology, the animal production license number is SCXK (E) 2016-0009, and the breeding environment temperature is 22 ~26°C, humidity 40~60%, mice free access to food and water.

[0068] 2. Drug solution preparation: yeast extract (batch number 20160628, purchased from Beijing Aoboxing Biotechnology Co., Ltd.), oxonic acid potassium salt (batch number P137112, purchased from Shanghai Aladdin Biochemical Technology Co., Ltd.) and allopurinol (batch number Y27O8C46913, purchased from Shanghai Yuanye Biotechnology Co., Ltd.), all adopt 0.5% CMC-Na aqueous solution as solvent, be mixed with the yeast extract suspension that yeast extract concentration is 3g / mL respectively, the potassium oxonate concentratio...

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Abstract

The invention discloses a constructing method for a subacute hyperuricemia renal damage mouse model. The method comprises the following steps that yeast cream and oteracil potassium are taken as a molding agent, a molding experiment is constantly carried out on an experiment mouse for multiple days, a subacute hyperuricemia renal damage mouse model is acquired, wherein during the molding experiment process, the yeast cream is given to the experiment mouse every day, and after the yeast cream is given to the experiment mouse in the last day of the molding experiment, the oteracil potassium is given to the experiment mouse; the weight, serum uric acid level, renal function change and renal pathology change of the experiment mouse are observed, and damage of the molding agent to the experiment mouse is analyzed. According to the constructing method, the mouse is taken as a model animal, the yeast cream in which a uric acid precusor substance is added for a long term is adopted, the oteracil potassium as a uricase inhibitor is taken as the molding agent and is used, and the subacute hyperuricemia renal damage mouse model with stable effect is in accordance with clinical features and issuccessfully constructed.

Description

technical field [0001] The invention relates to the technical field of animal models of hyperuricemia, in particular to a method for constructing a mouse model of subacute hyperuricemia with renal damage. Background technique [0002] Hyperuricemia is a type of disease in which the metabolism of purine in the body is abnormal or the function of the kidneys to excrete uric acid is weakened, resulting in excessive production or decreased excretion of uric acid in the body, which in turn leads to a high level of serum uric acid. If the fasting blood uric acid level exceeds 420 μmol / L for men and 360 μmol / L for women on two different days, it is called hyperuricemia, and long-term hyperuricemia often induces kidney damage. The results of investigation and research show that with the improvement of people's living standards, the incidence of hyperuricemia is increasing day by day, and the cases of hyperuricemia renal damage are also increasing. The current drugs for the treatmen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/064A61K31/53A61K49/00
CPCA61K31/53A61K36/064A61K49/0008A61K2300/00
Inventor 徐凌云裴忆雪刘永杰张笛刘德俊
Owner WUHAN POLYTECHNIC UNIVERSITY
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