High-activity wood frog anti-oxidation peptide oral liquid and preparation method thereof

A technology of oxidative peptide oral liquid and antioxidant peptide, which can be used in food ingredients as taste improver, function of food ingredients, food ingredients containing natural extracts, etc., can solve problems such as waste of resources, delay aging and enhance immunity , the effect of low foaming

Inactive Publication Date: 2018-09-18
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Rana is a high-quality animal resource in Jilin Province. After a comprehensive analysis of its utilization in recent years, it is found that most of the utilization is c

Method used

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  • High-activity wood frog anti-oxidation peptide oral liquid and preparation method thereof
  • High-activity wood frog anti-oxidation peptide oral liquid and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1 An anti-oxidation instant granule and its preparation method, the preparation method includes the following steps:

[0036] A1: Preparation of high-activity Rana chensinensis antioxidant peptide:

[0037] (a) Preparation of separation and purification sample solution

[0038] Weigh 10 g of Rana chensinensis polypeptide, dilute to 100 mL with ultrapure water, prepare a 100 mg / mL polypeptide solution, and store at 4°C for later use.

[0039] (b) Preparation of gel chromatography column

[0040] Take 100g of Sephadex G15 dry gel powder, add 800g of ultrapure water to dissolve it, boil on an electric heating furnace until it is fully swollen, let stand and cool to room temperature, stir the gel swelling liquid evenly, and pour it into the chromatography column (inner diameter 18mm) , Length 75cm), pay attention to no bubbles and no faults, make the height of the settled gel equal to 50cm, and keep the liquid level level. Using ultrapure water as the mobile phase, contr...

Embodiment 2

[0061] Embodiment 2 An antioxidant instant granule and its preparation method. The preparation method includes the following steps:

[0062] A2: Preparation of high activity Rana chensinensis antioxidant peptide:

[0063] (a) Preparation of separation and purification sample solution

[0064] Weigh 15 g of Rana chensinensis polypeptide, dilute to 150 mL with ultrapure water, prepare a 100 mg / mL polypeptide solution, and store at 4°C for later use.

[0065] (b) Preparation of gel chromatography column

[0066] Take 100g of SephadexG15 gel dry powder, add 1000g of ultrapure water to dissolve it, boil on an electric heating stove until fully swelled, let stand and cool to room temperature, stir the gel swelling liquid evenly, and continuously and slowly pour it into the chromatography column (inner diameter 18mm , Length 75cm), pay attention to no bubbles and no faults, make the height of the settled gel equal to 55cm, and keep the liquid level level. Using ultrapure water as the mobile ...

Embodiment 3

[0087] Embodiment 3 An antioxidant instant granule and its preparation method. The preparation method includes the following steps:

[0088] A3: Preparation of high activity Rana chensinensis antioxidant peptide:

[0089] (a) Preparation of separation and purification sample solution

[0090] Weigh 5 g of Rana chensinensis polypeptide, dilute to 50 mL with ultrapure water, prepare a 100 mg / mL polypeptide solution, and store at 4°C for later use.

[0091] (b) Preparation of gel chromatography column

[0092] Take 100g of Sephadex G15 gel dry powder, add 1200g of ultrapure water to dissolve, boil on an electric heating furnace until fully swelled, let stand and cool to room temperature, stir the gel swelling liquid evenly, and continuously and slowly pour into the chromatography column (inner diameter 18mm , Length 75cm), pay attention to no bubbles and no faults, make the height of the settled gel equal to 60cm, and keep the liquid level level. Using ultrapure water as the mobile phase...

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Abstract

The invention relates to a high-activity wood frog anti-oxidation peptide oral liquid and a preparation method thereof. The high-activity wood frog anti-oxidation peptide oral liquid is prepared through technologically treating high-activity wood frog anti-oxidation peptide with an extracted clarified hawthorn fruit juice and flavorings, such as sucrose and citric acid. The high-activity wood froganti-oxidation peptide is a component detected to have a highest anti-oxidation activity in three components separated and purified from dried powdered wood frog collagen peptides having a molecularweight of 3500 Da or below by Sephadex G15 gel chromatography, and the clarified hawthorn fruit juice is obtained by a raw material pretreatment and leaching technology. Data of detection of the DPPHclearance rate, the reducing power, the hydroxyl radical scavenging rate and the superoxide anion clearance rate of the oral liquid in in-vitro antioxidant experiments show that the oral liquid has considerable anti-oxidation properties.

Description

Technical field [0001] The invention belongs to the field of health drinks, and relates to an anti-oxidant oral liquid with a high-activity polypeptide as a functional component and a preparation method thereof, and is a public health drink that can be drunk when the lid is opened. Background technique [0002] Forest frog is a high-quality animal resource in Jilin Province. A comprehensive analysis of its utilization in recent years found that most of the use is concentrated on the research and development of forest frog oil and its products, and the wood frog remains as waste, resulting in huge resources. waste. Combining with the research hotspots of contemporary antioxidant health care products, a highly active Rana sylvatica anti-oxidant peptide oral liquid is made from the enzymatic hydrolysis product Rana sylvatica polypeptide extracted from Rana serrata residues as raw materials. The product is used to prevent diseases caused by free radicals, such as diabetes, cancer, a...

Claims

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Application Information

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IPC IPC(8): A23L2/02A23L2/52A23L33/18A23L33/105
CPCA23L2/02A23L2/52A23L33/105A23L33/18A23V2002/00A23V2200/30A23V2200/14A23V2200/16A23V2250/032A23V2250/21A23V2250/55A23V2250/628A23V2300/14
Inventor 奚春宇赵静张梦寒熊文欣康乃馨
Owner JILIN UNIV
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