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A double-pulse detection method of immune response based on mass spectrometry

An immune reaction and detection method technology, applied in the field of medical testing, can solve the problems of complicated operation of enzyme-linked immunosorbent assay and chemiluminescence method, large batch-to-batch variation of fluorescence immunochromatography, poor stability of markers, etc., so as to improve the system detection. Sensitivity, no washing process, high sensitivity effect

Active Publication Date: 2022-02-15
TIANJIN SAVANT BIOTECH CO LTD
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Problems solved by technology

However, radioimmunoassay has radioactive pollution, which is harmful to the human body; enzyme-linked immunoassay and chemiluminescent method are cumbersome to operate and have a long detection cycle; the electrode equipment used in the electrochemical detection system and the sophisticated signal analysis system are expensive. ; Colloidal gold method, fluorescence immunochromatography method has large batch-to-batch variability and poor stability of markers; microfluidic chip method has not yet achieved commercial application

Method used

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  • A double-pulse detection method of immune response based on mass spectrometry
  • A double-pulse detection method of immune response based on mass spectrometry
  • A double-pulse detection method of immune response based on mass spectrometry

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Experimental program
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Embodiment 1

[0042] Embodiment 1 (double pulse sandwich method)

[0043] 1. Preparation of Pt nanoparticles:

[0044] 0.15g polyvinylpyrrolidone (PVP), 33.9mg chloroplatinic acid (H 2 PtCl 6 .6H 2 (2), 20mL ultrapure water, and 20mL ethylene glycol were added to the reaction vessel, and under vigorous stirring, the reaction was refluxed at 60° C. for 3 h to obtain a brown-black colloidal solution of Pt nanoparticles.

[0045] 2. Streptavidin-labeled gold nanoparticles:

[0046] Take 0.5mL, 2.5mg / mL gold (Au) microspheres (150nm, Xi'an Kaixin Biotechnology Co., Ltd.) wash twice with PBS buffer solution (pH=7.4) and resuspend in 10mL of PBS buffer solution; Continue to add 5mg EDC and 5mg NHS to the washed gold microsphere solution, and activate it under stirring for 1h; wash with PBS buffer solution and resuspend in 10mL PBS buffer solution, continue to add blocking solution for blocking for 30min, wash with PBS buffer solution and resuspend Suspended in 10mL PBS buffer solution for us...

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Abstract

The invention discloses a double-pulse detection method of immune reaction based on mass spectrometry analysis technology. The double-pulse signal detection method is realized by using inductively coupled plasma or microwave plasma mass spectrometry technology. By using metal nanoparticles to mark immune reaction, further use The inductively coupled plasma or microwave plasma mass spectrometry technology detects the pulse signal of the metal ion that marks the immune reaction, so as to achieve the purpose of indirect detection of the analyte.

Description

technical field [0001] The invention belongs to the field of medical testing, but is not limited to this field, and specifically relates to a method for detecting double-pulse immune responses based on mass spectrometry analysis technology. Background technique [0002] At present, in terms of immunodiagnosis, radioimmunoassay, enzyme-linked immunoassay, chemiluminescence, and electrochemiluminescence are commonly used clinically; at the same time, in the field of rapid testing, colloidal gold method, fluorescent immunochromatography and emerging methods are commonly used. Microfluidic chip detection methods, etc. However, radioimmunoassay has radioactive pollution, which is harmful to the human body; enzyme-linked immunoassay and chemiluminescent method are cumbersome to operate and have a long detection cycle; the electrode equipment used in the electrochemical detection system and the sophisticated signal analysis system are expensive. ; The colloidal gold method and the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/543G01N33/533G01N33/577G01N33/68G01N27/62G01N27/64
CPCG01N27/626G01N27/64G01N33/533G01N33/54346G01N33/577G01N33/6893G01N2560/00G01N2333/4728
Inventor 林斯
Owner TIANJIN SAVANT BIOTECH CO LTD
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