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Immunoreaction double-pulse detection method based on mass spectrographic analysis technology

An immune reaction and detection method technology, which is applied in the field of medical testing, can solve the problems of cumbersome operation of enzyme-linked immunoassay and chemiluminescence method, large batch-to-batch variation of fluorescent immunochromatography, and poor stability of markers, etc., to improve system detection Sensitivity, stable detection results, and high sensitivity

Active Publication Date: 2018-09-28
TIANJIN SAVANT BIOTECH CO LTD
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Problems solved by technology

However, radioimmunoassay has radioactive pollution, which is harmful to the human body; enzyme-linked immunoassay and chemiluminescent method are cumbersome to operate and have a long detection cycle; the electrode equipment used in the electrochemical detection system and the sophisticated signal analysis system are expensive. ; Colloidal gold method, fluorescence immunochromatography method has large batch-to-batch variability and poor stability of markers; microfluidic chip method has not yet achieved commercial application

Method used

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  • Immunoreaction double-pulse detection method based on mass spectrographic analysis technology
  • Immunoreaction double-pulse detection method based on mass spectrographic analysis technology
  • Immunoreaction double-pulse detection method based on mass spectrographic analysis technology

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Embodiment 1

[0042] Embodiment 1 (double pulse sandwich method)

[0043] 1. Preparation of Pt nanoparticles:

[0044] 0.15g polyvinylpyrrolidone (PVP), 33.9mg chloroplatinic acid (H 2 PtCl 6 .6H 2 (2), 20mL ultrapure water, and 20mL ethylene glycol were added to the reaction vessel, and under vigorous stirring, the reaction was refluxed at 60° C. for 3 h to obtain a brown-black colloidal solution of Pt nanoparticles.

[0045] 2. Streptavidin-labeled gold nanoparticles:

[0046] Take 0.5mL, 2.5mg / mL gold (Au) microspheres (150nm, Xi'an Kaixin Biotechnology Co., Ltd.) wash twice with PBS buffer solution (pH=7.4) and resuspend in 10mL of PBS buffer solution; Continue to add 5mg EDC and 5mg NHS to the washed gold microsphere solution, and activate it under stirring for 1h; wash with PBS buffer solution and resuspend in 10mL PBS buffer solution, continue to add blocking solution for blocking for 30min, wash with PBS buffer solution and resuspend Suspended in 10mL PBS buffer solution for us...

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Abstract

The invention discloses an immunoreaction double-pulse detection method based on a mass spectrographic analysis technology. The double-pulse detection method is realized by using an inductive couplingplasma or microwave plasma mass spectrometry technology; by using metal nanometer particle labeling immunoreaction, the inductive coupling plasma or microwave plasma mass spectrometry technology is further used for detecting and labeling the metal ion pulse signals of the immunoreaction, so that the goal of indirectly detecting objects to be detected can be achieved.

Description

technical field [0001] The invention belongs to the field of medical testing, but is not limited to this field, and specifically relates to a method for detecting double-pulse immune responses based on mass spectrometry analysis technology. Background technique [0002] At present, in terms of immunodiagnosis, radioimmunoassay, enzyme-linked immunoassay, chemiluminescence, and electrochemiluminescence are commonly used clinically; at the same time, in the field of rapid testing, colloidal gold method, fluorescent immunochromatography and emerging methods are commonly used. Microfluidic chip detection methods, etc. However, radioimmunoassay has radioactive pollution, which is harmful to the human body; enzyme-linked immunoassay and chemiluminescent method are cumbersome to operate and have a long detection cycle; the electrode equipment used in the electrochemical detection system and the sophisticated signal analysis system are expensive. ; The colloidal gold method and the...

Claims

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Application Information

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IPC IPC(8): G01N33/543G01N33/533G01N33/577G01N33/68G01N27/62G01N27/64
CPCG01N27/626G01N27/64G01N33/533G01N33/54346G01N33/577G01N33/6893G01N2560/00G01N2333/4728
Inventor 林斯
Owner TIANJIN SAVANT BIOTECH CO LTD
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