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A metal ion-labeled immunoreaction double-pulse detection method

An immune reaction and metal ion technology, applied in the field of medical testing, can solve the problems of cumbersome operation of enzyme-linked immunoassay and chemiluminescence method, large batch-to-batch variation of fluorescent immunochromatography, and poor stability of markers, etc., to improve system detection Effect of sensitivity, high sensitivity, and low detection speed

Active Publication Date: 2022-02-15
TIANJIN SAVANT BIOTECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, radioimmunoassay has radioactive pollution, which is harmful to the human body; enzyme-linked immunoassay and chemiluminescent method are cumbersome to operate and have a long detection cycle; the electrode equipment used in the electrochemical detection system and the sophisticated signal analysis system are expensive. ; Colloidal gold method, fluorescence immunochromatography method has large batch-to-batch variability and poor stability of markers; microfluidic chip method has not yet achieved commercial application

Method used

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  • A metal ion-labeled immunoreaction double-pulse detection method
  • A metal ion-labeled immunoreaction double-pulse detection method
  • A metal ion-labeled immunoreaction double-pulse detection method

Examples

Experimental program
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Effect test

Embodiment 1

[0051] Embodiment 1 (double pulse competition method)

[0052] 1. Preparation of Pt nanoparticles:

[0053] 0.15g polyvinylpyrrolidone (PVP), 33.9mg chloroplatinic acid (H 2 PtCl 6 .6H 2 (2), 20mL ultrapure water, and 20mL ethylene glycol were added to the reaction vessel, and under vigorous stirring, the reaction was refluxed at 60° C. for 3 h to obtain a brown-black colloidal solution of Pt nanoparticles.

[0054] 2. Streptavidin-labeled gold nanoparticles:

[0055] Take 0.5mL, 2.5mg / mL gold (Au) microspheres (150nm, Xi'an Kaixin Biotechnology Co., Ltd.) wash twice with PBS buffer solution (pH=7.4) and resuspend in 10mL of PBS buffer solution; Continue to add 5mg EDC and 5mg NHS to the washed gold microsphere solution, and activate it under stirring for 1h; wash with PBS buffer solution and resuspend in 10mL PBS buffer solution, continue to add blocking solution for blocking for 30min, wash with PBS buffer solution and resuspend Suspended in 10mL PBS buffer solution for...

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Abstract

The invention discloses a metal ion-labeled immune reaction double-pulse detection method. The double-pulse signal detection method is realized by using inductively coupled plasma or microwave plasma mass spectrometry analysis technology. By using metal nanoparticles to mark the immune reaction, the inductance Coupled plasma or microwave plasma mass spectrometry technology detects the pulse signal of the metal ion that marks the immune reaction, so as to achieve the purpose of indirect detection of the analyte.

Description

technical field [0001] The invention belongs to the field of medical testing, but is not limited to this field, and specifically relates to a metal ion-labeled immune reaction double-pulse detection method. Background technique [0002] At present, in terms of immunodiagnosis, radioimmunoassay, enzyme-linked immunoassay, chemiluminescence, and electrochemiluminescence are commonly used clinically; at the same time, in the field of rapid testing, colloidal gold method, fluorescent immunochromatography and emerging methods are commonly used. Microfluidic chip detection methods, etc. However, radioimmunoassay has radioactive pollution, which is harmful to the human body; enzyme-linked immunoassay and chemiluminescent method are cumbersome to operate and have a long detection cycle; the electrode equipment used in the electrochemical detection system and the sophisticated signal analysis system are expensive. ; The colloidal gold method and the fluorescent immunochromatography ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/53G01N27/62G01N27/64
CPCG01N27/62G01N27/64G01N33/53
Inventor 林斯
Owner TIANJIN SAVANT BIOTECH CO LTD
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