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Stimulant for plant grafting propagation and preparation method thereof

A stimulant and plant technology are applied in the field of stimulants for plant grafting and propagation and the field of preparation thereof, which can solve the problems of difficulty in disinfection, difficulty in tissue culture and genetic transformation, and achieve a slight increase in the growth amount of fringes and the number of lateral buds, and improve healing. Injured tissue vitality, reasonable formula effect

Active Publication Date: 2018-10-02
福建山瓜瓜农业发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Third, adult plants carry bacteria and fungi during their growth, making disinfection difficult. This has brought difficulties to tissue culture and genetic transformation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A stimulant for plant grafting and reproduction, which includes plant hormones and fungi;

[0030] Specifically prepared according to the following process:

[0031] Streak inoculation of Fusarium chlorosporum on a PDA medium to obtain a single colony; pick a single colony to inoculate on a seed medium and cultivate it at 30°C for 24 hours to obtain a seed solution of Fusarium chlorosporum for use; The formula of the culture medium is: glucose 10g / L, yeast extract 6g / L, dipotassium hydrogen phosphate 1g / L, potassium dihydrogen phosphate 1g / L, magnesium sulfate 0.01g / L, ferrous sulfate 0.01g / L;

[0032] Inoculate Cladosporium cladosporium on a PDA medium and culture to obtain a single colony; pick a single colony to inoculate the PDA liquid medium and cultivate it at 30°C for 24 hours to obtain a cladosporium cladosporium seed solution, and set aside;

[0033] The seed liquid of Fusarium chlamydosporium and Cladosporium cladosporum were successively connected to the MS medium, a...

Embodiment 2

[0036] A stimulant for plant grafting and reproduction, which includes plant hormones and fungi;

[0037] Specifically prepared according to the following process:

[0038] Streak inoculation of Fusarium chlorosporum on a PDA medium to obtain a single colony; pick a single colony to inoculate on a seed medium and cultivate it at 30°C for 24 hours to obtain a seed solution of Fusarium chlorosporum for use; The formula of the culture medium is: glucose 10g / L, yeast extract 6g / L, dipotassium hydrogen phosphate 1g / L, potassium dihydrogen phosphate 1g / L, magnesium sulfate 0.01g / L, ferrous sulfate 0.01g / L;

[0039] Inoculate Cladosporium cladosporium on a PDA medium and culture to obtain a single colony; pick a single colony to inoculate the PDA liquid medium and cultivate it at 30°C for 24 hours to obtain a cladosporium cladosporium seed solution, and set aside;

[0040] The seed liquid of Fusarium chlamydosporium and Cladosporium cladosporium were successively connected to MS medium, the ...

Embodiment 3

[0043] A stimulant for plant grafting and reproduction, which includes plant hormones and fungi;

[0044] Specifically prepared according to the following process:

[0045] Streak inoculation of Fusarium chlorosporum on a PDA medium to obtain a single colony; pick a single colony to inoculate on a seed medium and cultivate it at 30°C for 24 hours to obtain a Fusarium chlorosporum seed solution for use; The formula of the culture medium is: glucose 10g / L, yeast extract 6g / L, dipotassium hydrogen phosphate 1g / L, potassium dihydrogen phosphate 1g / L, magnesium sulfate 0.01g / L, ferrous sulfate 0.01g / L;

[0046] Inoculate cladosporium cladosporium on PDA medium and cultivate to obtain a single colony; pick a single colony to inoculate on PDA liquid medium and cultivate it at 30°C for 24 hours to obtain cladosporium cladosporium seed solution, and set aside;

[0047] The seed solution of Fusarium chlamydosporium and Cladosporium cladosporum were successively connected to MS medium, the inocu...

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PUM

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Abstract

Belonging to the technical field of plant grafting propagation, the invention discloses a stimulant for plant grafting propagation. The stimulant comprises fungi and plant hormones. According to the invention, traditional plant growth hormones and microorganisms are combined to prepare the novel stimulant, the stimulant has the characteristics of reasonable formula, synergistic interaction and convenient use, and can enhance the survival rate of scions in grafting.

Description

Technical field [0001] The invention belongs to the technical field of plant grafting and propagation, and specifically relates to a stimulant for plant grafting and propagation and a preparation method thereof. Background technique [0002] Grafting refers to the technique of transferring the buds and branches of a good variety of plants to the stems and roots of another plant body, and healing them to form a new independent plant. It is a technique widely used in the process of horticultural crop seedling raising and production. The biological principle of grafting survival is that on the surface of the cut surface of the stock, the residue of dead cells forms a brown septum. Then, due to the action of the callus, the cells around the wound divide and the cambium cells are active and break through the septum to form healing. The wound tissue and callus cells further differentiate to form a new xylem inward and a new phloem outward to communicate the respective xylem ducts of th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/04A01N43/90A01N37/10A01N37/12A01P21/00
CPCA01N37/10A01N37/12A01N43/90A01N63/30
Inventor 潘文鋆赵志华赵成龙吕伟红吕伟德
Owner 福建山瓜瓜农业发展有限公司
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