A kind of preparation method of SERS chip
A chip, pit technology
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Embodiment 1
[0061] Preparation of nano-sol:
[0062] Add 1mL of 1% silver nitrate aqueous solution into a 100mL three-necked flask and dilute to 100ml; heat the solution to boiling, and add 1% sodium citrate solution under constant reflux and vigorous stirring. The solution gradually changed from colorless to light blue. Start timing after discoloration, keep the system in a boiling state for 15 minutes under stirring, and then naturally cool to room temperature to obtain silver sol.
Embodiment 2
[0064] Preparation of nano-sol:
[0065] In a 100mL three-necked flask, add 1mL of an aqueous solution with a mass concentration of 1% silver nitrate and chloroauric acid (feeding molar ratio is 1:4) and dilute to 100ml; heat the solution to boiling, and add the concentration under continuous reflux and vigorous stirring. 1% sodium citrate solution. The solution gradually turned from light yellow to wine red. Start timing after discoloration, keep the system in a boiling state for 15 minutes under agitation, and then naturally cool to room temperature to obtain a gold-silver alloy sol.
Embodiment 3
[0067] Preparation of nano-sol:
[0068] In a 100mL three-necked flask, add 1mL of 1% chloroauric acid aqueous solution and dilute to 100ml; heat the solution to boiling, and add 1% sodium citrate solution under continuous reflux and vigorous stirring. The solution gradually turned from light yellow to wine red. Start timing after discoloration, keep the system in a boiling state for 15 minutes under stirring conditions, and then naturally cool to room temperature to obtain gold sol.
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