60Co-gamma ray radiation mutagenesis method for populus euphratica seeds treated with salt stress

A technology of salt stress and γ-rays, applied in the field of radiation breeding, can solve the problem of less application, and achieve the effect of reducing the relative salt damage rate and good tolerance

Inactive Publication Date: 2018-10-16
INST OF AFFORESTATION & DESERTIFICATION PREVENTION & CONTROL XINJIANG ACADEMY OF FOREST SCI
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  • Abstract
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Problems solved by technology

Radiation mutation breeding has been used in other plant breeding in my country, but it is rarely used in plants such as Populus eu

Method used

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  • 60Co-gamma ray radiation mutagenesis method for populus euphratica seeds treated with salt stress
  • 60Co-gamma ray radiation mutagenesis method for populus euphratica seeds treated with salt stress
  • 60Co-gamma ray radiation mutagenesis method for populus euphratica seeds treated with salt stress

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Embodiment 1

[0021] Populus euphratica seeds provided by the invention 60 Co-γ-ray radiation mutagenesis method, the specific embodiment is:

[0022] Select Populus euphratica seeds, including steps:

[0023] A. Culture conditions:

[0024] Seed salt stress treatment: the salt was set to NaCl solution, and the concentration was set to 0 (CK), 25, 50, 150, 250, 300 mmol / L, a total of 6 salt stress treatments, including 1 control treatment.

[0025] The above culture conditions are temperature 25-27°C, light intensity 3 000lx; light-dark cycle 16 / 8h.

[0026] B. Radiation treatment:

[0027] Populus euphratica seeds are used as test material, for grouping 60 Co-γ rays for radiation treatment;

[0028] 60 Co-γ-ray radiation dose (0, 50, 100, 150, 200Gy), irradiated seeds with 0.1% HgCl 2 Disinfect for 5-7 minutes, rinse with distilled water 3 times for 1 minute each time. The test was set at 0 (CK, distilled water), 25, 50, 150, 250 and 300mmol·L -1 NaCl concentration gradient, pour ...

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Abstract

The invention discloses a 60Co-gamma ray radiation mutagenesis method for populus euphratica seeds treated with salt stress. The method comprises the steps as follows: the populus euphratica seeds areirradiated with a 60Co-gamma ray, and then salt stress treatment of the populus euphratica seeds is carried out by adopting a sodium chloride solution. The method has the benefits that: after the operation according to the radiation mutagenesis method provided by the invention, the germination rate, the germination potential and the relative germination rate of 150 Gy dose radiation mutagenesis are significantly higher than that receiving other radiation treatments, and the relative salt harm rate is lowered. By establishing the 60Co-gamma ray radiation mutation breeding technology of the populus euphratica seeds, the basis for the selection and the determination of the optimal radiation dose for radiation mutation breeding is provided, the germination mechanism of the populus euphraticaseeds against radiation mutagenesis and NaCl stress is revealed, the appropriate radiation dose for salt resistance of seeds is provided, and new methods and approaches for planting resources innovation and variety breeding of populus euphratica are further provided through the radiation mutation breeding.

Description

technical field [0001] The invention relates to the technical field of radiation breeding, in particular to the production of Populus euphratica seeds used for salt stress treatment. 60 Co-γ-ray radiation mutagenesis method. Background technique [0002] Radiation mutagenesis (physical mutagenesis) breeding is the use of seed radiation technology for breeding. It is a technology with high breeding efficiency at present. It mainly treats materials with a certain dose of radiation to induce mutants of material genes or aberrations in chromosomes. After screening , Determination, and selection, so as to obtain mutants in a short period of time and select new varieties from them. [0003] Radiation mutagenesis is the process of using a certain dose of radiation to irradiate plant plants, pollen, seeds or organs to induce mutations and make them produce favorable mutations. New varieties are bred through artificial screening and cultivation. The premise of radiation mutagenesis...

Claims

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Application Information

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IPC IPC(8): A01H1/06
CPCA01H1/06
Inventor 伊丽米努尔阿不都热西提·热合曼阿力比亚提·玉素甫宁虎森卡依拉·阿布都艾尼早尔古·吐逊江艾力江·麦麦提阿卜杜许库尔·牙合甫米热古丽·亚森阿衣夏木·亚库甫
Owner INST OF AFFORESTATION & DESERTIFICATION PREVENTION & CONTROL XINJIANG ACADEMY OF FOREST SCI
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