60Co-gamma ray radiation mutagenesis method for populus euphratica seeds treated with salt stress

Abstract

The invention discloses a 60Co-gamma ray radiation mutagenesis method for populus euphratica seeds treated with salt stress. The method comprises the steps as follows: the populus euphratica seeds areirradiated with a 60Co-gamma ray, and then salt stress treatment of the populus euphratica seeds is carried out by adopting a sodium chloride solution. The method has the benefits that: after the operation according to the radiation mutagenesis method provided by the invention, the germination rate, the germination potential and the relative germination rate of 150 Gy dose radiation mutagenesis are significantly higher than that receiving other radiation treatments, and the relative salt harm rate is lowered. By establishing the 60Co-gamma ray radiation mutation breeding technology of the populus euphratica seeds, the basis for the selection and the determination of the optimal radiation dose for radiation mutation breeding is provided, the germination mechanism of the populus euphraticaseeds against radiation mutagenesis and NaCl stress is revealed, the appropriate radiation dose for salt resistance of seeds is provided, and new methods and approaches for planting resources innovation and variety breeding of populus euphratica are further provided through the radiation mutation breeding.

Description

technical field [0001] The invention relates to the technical field of radiation breeding, in particular to the production of Populus euphratica seeds used for salt stress treatment. 60 Co-γ-ray radiation mutagenesis method. Background technique [0002] Radiation mutagenesis (physical mutagenesis) breeding is the use of seed radiation technology for breeding. It is a technology with high breeding efficiency at present. It mainly treats materials with a certain dose of radiation to induce mutants of material genes or aberrations in chromosomes. After screening , Determination, and selection, so as to obtain mutants in a short period of time and select new varieties from them. [0003] Radiation mutagenesis is the process of using a certain dose of radiation to irradiate plant plants, pollen, seeds or organs to induce mutations and make them produce favorable mutations. New varieties are bred through artificial screening and cultivation. The premise of radiation mutagenesis...

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Problems solved by technology

Radiation mutation breeding has been used in other plant breeding in my country, but it is rarely used in plants such as Populus euphratica seeds

Moreover, few scholars have used NaCl stress and 60 Study on Populus euphratica Seeds Treated with Co-γ Ray Radiation

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